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4-1BB

Name: 4-1BB
SKU: ASHU-00099
Availability: InStock

$0.00

SKU: ASHU-00099

Category: Immune Checkpoint Mouse Models

Description
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Knock-in Strategy

Figure 1. Gene knock-in strategy to engineer a mouse model expressing a chimeric human-murine 4-1BB (also called CD137, TNFRSF9) gene. The endogenous mouse Tnfrsf9 gene was replaced with a human TNFRSF9 extracellular and mouse Tnfrsf9 transmembrane-intracellular (cytoplasmic) region downstream of the ATG start codon.

4-1BB (CD137) Expression in Humanized Mouse Model

Figure 2. Flow cytometric analysis of pan T cells isolated from male heterozygous humanized 4-1BB mice (hCD137+/-). Male spleen pan T cells were isolated and stimulated with 1µg/mL of anti-CD3 and 1µg/mL of anti-CD28 for 48 hours. The cells were stained with anti-hCD137-APC, mCD137-PE, mCD3-V450 and mCD8-FITC for flow cytometry analysis. Results showed that human 4-1BB (hCD137) was expressed in both activated CD4+ and CD8+ lymphocytes.

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Material Transfer Agreement

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This Material Transfer Agreement (“MTA”) is between you (“Purchaser”) and the Applied StemCell, Inc. a California company, having its principal place of business at 521 Cottonwood Drive Suite 111, Milpitas, CA 95035 USA (“ASC”). Purchaser must have an approved, current ASC account to place an order. This MTA is effective for a period of five (5) years as of the last date of execution by the parties and governs the purchase and use of all ASC Materials under the terms and conditions set forth below.

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4-1BB

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Knock-in Strategy

4-1BB (CD137) Expression in Humanized Mouse Model

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CD86

BTLA (2)

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4-1BB

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Knock-in Strategy

4-1BB (CD137) Expression in Humanized Mouse Model

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CD86

BTLA (2)

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MTA Agreement

Material Transfer Agreement

TERMS AND CONDITIONS

Definitions

Scope of Use

Warranty; Warranty Disclaimer

Limitation of Liability