APOE4

Humanized Strategy

Validation Data

Figure 1. Genotyping of WT, KO &  Humanized mice by PCR. Primer P1/2 Wild type = 342 bp Primer P3/4 mutant = 332 bp M：100bp Plus DNA ladder.

Figure 2. Western blot analysis of  APOE4 protein from WT, KO & Humanized mice.  APOE anti-body: Sc-13521 (santa cruz)

Validate Human APOE Expression by Western Blot

KO: Apoe knockout mouse sample; hAPOE2 : humanized APOE2 mouse sample (ASHU-190013); hAPOE3 : humanized APOE3 mouse sample (ASHU-190003); hAPOE4 : humanized APOE4 mouse sample (ASHU-190002); WT: wild type mouse sample; M: marker.