iPSC-Derived Human Cortical Neuron Kit (African-American, Male Line)
Applied StemCell has developed an efficient integration-free method to differentiate high-quality cortical neurons from human iPSCs. The differentiated cortical neurons recapitulate the phenotype and functional parameters of primary and in vivo cortical neurons. We provide cortical neurons differentiated from an integration-free, control human iPSC line (ASE9211), reprogrammed from the fibroblasts of an African-American male donor. These high-purity (≥80%) cells express high levels of the cortical neuron biomarker Tuj1 (Figure 1). To harness the full potential of our cortical neurons, we also provide optimized Cortical Neuron Culture Media (ASE-9741MM) that supports robust maintenance and functionality of the cortical neurons in culture. These iPSC-differentiated cortical neurons can be used as control lines to compare phenotype and functionality of patient-derived, genome edited iPSC-derived cortical neurons for drug screening applications. These cortical neurons can also be used as effector cells for cytotoxicity assays.

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