Cd8a-IRES-Luc-2A-EGFP

A V5-IRES-luci-2A-EGFP expression cassette was inserted before the stop codon TAA of mouse Cd8a gene to establish a multi-labeled knock-in model with V5, luciferase and EGFP fluorescent protein.

Vallidation Data

Figure 1.  In the absence of any stimulation, the in vivo imaging system detected that Cd8a^{V5-IRES-luci-2A-EGFP/+} mice showed significant fluorescence. 

Figure 2.  In vivo imaging validation of MC38 tumors in Cd8a^{V5-IRES-luci-2A-EGFP/+} reporter mice treated or not with a PD-1 mAb. After 1 day of stimulation with PD1 antibody, the fluorescence was significantly enhanced.

Figure 3.  EGFP expression analysis in  PBMC of Cd8a^{V5-IRES-luci-2A-EGFP/+} mice by flow cytometry.