PD-1 & CTLA4

Generation Strategy

Strategy for Generating Humanized PD-1 (ASHU-00015) Mouse Model

Strategy for Generating Humanized CTLA4 (ASHU-00014) Mouse Model

The Expression of hPD-1 in Stimulated T Cells from Double Humanized PD-1 & CTLA4 Mice 

Figure 1. The expression of human CTLA-4 in double humanized PD-1 & CTLA-4 mice was confirmed by FACS.

Figure 2. In vivo validation of double humanized PD-1 & CTLA-4 mice. The Double humanized mice were inoculated with MC38 cells, and randomly assigned to different groups (n=8) when the tumor grew to a volume of 100 mm3. A combinatorial treatment of anti-CTLA-4 and anti-PD-1 demonstrated a noticeable improvement in efficacy as compared to the same dose of single agent (top figure) without affecting the animal body weight (bottom).

Figure 3. As shown in the previous figure, animals of group 1-4 received different doses of either single agent or combinatorial treatment. This graph showed the progression of the tumor implanted in the cured, G4 mice, implicating a systematic anti-tumor effect induced by the combinatorial antibody treatment.