ASE-9710

iPSC-Derived Human Retinal Pigment Epithelium (RPE) Kit (African-American, Male Line)

Applied StemCell has developed an efficient integration-free, small molecule-based method to differentiate high-quality retinal pigment epithelium (RPE) precursor cells from human iPSCs. The differentiated RPE cells recapitulate the phenotype and functional parameters of primary and in vivo RPE cells. We provide RPE cells differentiated from an integration-free, control human iPSC line (ASE-9211), reprogrammed from the fibroblasts of an African-American male donor. These high-purity (≥90%) cells express high levels of RPE biomarkers, PMEL1, MITF, ZO-1 and RPE65 (Figure 1). To harness the full potential of our RPE cells, we also provide optimized RPE Culture Media (ASE9710MM) that supports robust maintenance and functionality of the RPE cells in culture. These iPSC-differentiated RPE cells can be used as control lines to compare phenotype and functionality of patient-derived, genome edited iPSC-derived RPE cells for drug screening applications. These RPE cells can also be used as effector cells for cytotoxicity assays.