iPSC-Derived Human Astrocytes Kit (African-American, Male Line) kit
Applied StemCell has developed an efficient integration-free, small molecule-based method to differentiate high-quality astrocytes from human iPSCs. The differentiated astrocytes recapitulate the phenotype and functional parameters of primary and in vivo astrocytes. We provide astrocytes differentiated from an integration-free, control human iPSC line (ASE-9211), reprogrammed from the fibroblasts of an African-American male donor. These high-purity (≥95%) cells express high levels of astrocyte biomarkers, GFAP and s100beta (Figure 2). To harness the full potential of our astrocytes, we also provide optimized Astrocyte Basal Culture Media (ASE-9743MM) and Astrocyte Culture Media Supplement A (100X) (ASE-9743MM-A) that support the robust maintenance and functionality of the astrocytes in culture. These iPSC-differentiated astrocytes can be used as control lines to compare phenotype and functionality of patient-derived, genome edited iPSC-derived astrocytes for drug screening applications. These astrocytes can also be used in neurotoxicity assays.

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