A CreERT2-Wpre-polyA expression cassette was knocked into initiation codon ATG site of the Krt14 gene via CRISPR/Cas mediated recombination.

Validation Data

landingpage-aski-190024-Krt14-CreERT2-ki-mouse-1

 

Figure 1: CreERT2-mediated recombination in Skin, Tongue & Esophagus of Krt14 CreERT2+/-; R26 tdTomato+/- mice.

A. Krt14-CreERT2+/- ; Rosa26 tdTomato+/- ; Tamoxifen

B. Krt14-CreERT2+/-; Rosa26 tdTomato+/- 

C. Rosa26 tdTomato+/-

landingpage-aski-190024-Krt14-CreERT2-ki-mouse-2

Figure 2: Confocal images showing expression of tdTomato in Skin, Tongue & Esophagus of Krt14 CreERT2+/-; R26 tdTomato +/- mice.

A. Krt14-CreERT2+/- ; Rosa26 tdTomato+/- ; Tamoxifen

B. Krt14-CreERT2+/-; Rosa26 tdTomato+/- 

C. Rosa26 tdTomato+/-

Size

Mouse

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