IL18BP

The endogenous mice Il18bp gene was replaced by human IL18BP gene.

Validation Data

Figure1. Detection of IL18BP expression in thymus and liver by RT-PCR. 

Wild type: only one band at 354 bp with primers F1/R1 (mIl18bp);Heterozygous: one band at 354 bp with primers F1/R1 (mIl18bp) and one band at 220 bp with primers F2/R2 (hIL18BP); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

Figure 2. Detection of IL18BP expression in thymus and liver by RT-PCR. 

Wild type: only one band at 354 bp with primers F1/R1(mIl18bp); Homozygous: only one band at 220 bp with primers F2/R2(hIL18BP);  Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

Figure 3. Detection of hIL18BP expression in serum by ELISA. 

Abbr. HO, homozygous; HE, heterozygous; WT, wild type.

Figure 4. Detection of mIL18BP expression in serum by ELISA. 

Abbr. HO, homozygous; HE, heterozygous; WT, wild type.

Figure 5. Detection of hIL18BP expression in serum by ELISA (n=3). 

Abbr. HO, homozygous; HE, heterozygous; WT, wild type.

Figure 6. Detection of mIL18BP expression in serum by ELISA.

Abbr. HO, homozygous; HE, heterozygous; WT, wild type.