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iPSC Differentiation Services

Discover Our iPSC Differentiated Cell Services

Lineage-committed Cell Lines

Non-integrating Differentiation

Drug Discovery & Toxicity Screening

With the convenience of cell line models and the biorelevance of primary cells, iPSC-differentiated cell lines are an ideal, cutting-edge resource for disease modeling, drug discovery and screening, and regenerative medicine. Applied StemCell (ASC) offers comprehensive customized service to differentiate iPSCs to many different progenitor and lineage-committed cell types (neural stem cells, cardiomyocytes, RPE, hematopoietic Progenitors, and more), thereby expanding the scope of your research, drug discovery, or drug screening projects. Our custom iPSC differentiation service offers:

  • High efficiency, non-integrating differentiation protocols for different lineages
  • High Purity, progenitor and lineage-committed cell lines as determined by lineage-specific biomarkers
  • Differentiation from healthy/disease iPSC lines: customer cell lines, ASC’s control iPSC lines, or genetically engineered iPSCs
  • Isogenic cell line model development from parent iPSC line
  • Development of co-culture and organoid (islet organoid) models with multiple lineages is available
  • Fast turnaround time
  • GMP iPSC Differentiation Services Available  >> Learn More 

Our experts can differentiate your iPSCs or you can select from our inventory of well-characterized iPSC control lines. ASC even offers upstream iPSC generation from human or non-human samples and high-throughput CRISPR genome editing services. To learn more about our affordable, custom iPSC services contact us today.

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iPSC Differentiation Service Categories:

We specialize in many different lineages: neural, hematopoietic, cardiovascular, photo receptors and more. See our not all-inclusive list of lineages we offer. Don’t see a lineage you are interested in? We also offer customized differentiation protocol development for lineages not listed.

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  • Natural Killer Cells
  • Monocytes
  • Macrophage
  • Sensory Neurons
  • Microglia
  • GABAergic Neurons
  • Skeletal Muscle
  • Intestinal Organoid
  • T Cells
  • Neural Stem Cells
  • Dopaminergic Neurons
  • Oligodendrocytes
  • Cholinergic Neuron
  • Endothelial Cells
  • Retinal Pigment Epithelium
  • Hematopoietic Progenitor Cells
  • Astrocytes
  • Motor Neurons
  • Excitatory Neurons
  • Cardiomyocytes
  • Hepatocytes
  • Photoreceptor Cells

Applications for iPSC-derived Cells

iPSC-derived, lineage committed cells offer a novel resource for drug discovery and drug screening processes that is gaining immense popularity. Considered as a more biorelevant with promising translational value, they offer a high-throughput model for toxicity and safety pharmacology assessments, disease modeling and proof of concept experiments, and for cell therapy applications. Neural lineage cells are especially advantageous for early, high-throughput neurotoxicity screening of compounds reducing attrition rate.

Need a CRO for screening your compounds for neuro / cardio toxicity and drug screening? Explore our preclinical services platform.

We also offer cGMP compliant iPSC Differentiation Service >> Learn More

  • Case Studies

    Neuronal iPSC-Derived Cells

    Neural Progenitor Cells (NPCs)
    Case Study 1

    Figure 1. Immunostaining of the ASE-9740 Neural Progenitor Cells derived from Applied StemCell’s control iPSC line ASE-9211. The neural progenitor cells (NPCs) (Left, Red: Nestin; Blue: DAPI) can be further differentiated into neurons (Middle, Red: Tuj1; Blue: DAPI) and astrocytes (Right, Red: GFAP; Blue: DAPI).

    Astrocytes

    Case Study 1
    Project Description:

    • To differentiate astrocytes from Applied StemCell’s NIST Control Line, ASE-9211

    Timeline:

    1. iPSC Recovery & Expansion
    2. Astrocyte Differentiation
    3. Antibody Staining (GFAP+, S100beta) (Flow Cytometry is Available if Inquired)

    Turnaround time: 3-4 months

    ASE-9743-Bright Field Image of iPSC-derived Astrocytes From Customer-provided iPSCsFigure 1. Bright Field Image of iPSC-derived Astrocytes from Applied StemCell’s (ASC’s) Control Line, ASE-9211.

     

     

     

     

     


    ASE-9743-Antibody Staining of the Astrocytes differentiated from the NIST Control Line, ASE-9211.Figure 2: Antibody Staining of the Astrocytes differentiated from the NIST Control Line, ASE-9211. The astrocytes differentiated from the ASC’s iPSC line were stained with the astrocyte markers GFAP and s100beta. Left Three Images: Top Image: GFAP (Green), Middle Image: DAPI (Blue), Bottom Image: GFAP (Green) & DAPI (Blue); Right Three Images: Top Image: s100beta (Red), Middle Image: DAPI (Blue), Bottom Image: s100beta (Red) & DAPI (Blue)

     

     

     

     

     

     

     

     

     

    Cortical Neurons

    Case Study 1
    Characterization of the ASE-9741 Cortical Neurons

     

     

     

     

     

    Figure 1. Immunostaining of ASE-9741 iPSC-derived Cortical Neurons for a cortical neuron biomarker. Cryopreserved cortical neurons, differentiated from Applied StemCell’s control iPSC line ASE-9211, were stained with Tuj1. (Red: Tuj1; Blue: DAPI)

    Dopaminergic Neurons

    Case Study 1
    Immunostaining of the ASE-9742 Dopaminergic Neurons derived from the human iPSC control line ASE-9211

     

     

     

     


    Figure 1. Immunostaining of the ASE-9742 Dopaminergic Neurons derived from the human iPSC control line ASE-9211.     The progenitors were further differentiated into TH+ dopaminergic neurons in 3 weeks. The dopaminergic neurons were identified by TH.

    Immunostaining of the ASE-9742 Dopaminergic Progenitors.

     

     

     

     

     


    Figure 2.     Immunostaining of the ASE-9742 Dopaminergic Progenitors. The dopaminergic progenitors were identified by Corin, OTX2, and Nestin.

  • Publications

    • Gupta, G., Gliga, A., Hedberg, J., Serra, A., Greco, D., Odnevall Wallinder, I., & Fadeel, B. Cobalt nanoparticles trigger ferroptosis‐like cell death (oxytosis) in neuronal cells: Potential implications for neurodegenerative disease. The FASEB Journal.
    • Kussauer, S., David, R., & Lemcke, H. (2019). hiPSCs Derived Cardiac Cells for Drug and Toxicity Screening and Disease Modeling: What Micro-Electrode-Array Analyses Can Tell Us. Cells, 8(11), 1331.
    • Cheng, F., Fransson, L. Å., & Mani, K. (2019). The cyanobacterial neurotoxin β-N-methylamino-l-alanine prevents addition of heparan sulfate to glypican-1 and increases processing of amyloid precursor protein in dividing neuronal cells. Experimental Cell Research. https://doi.org/10.1016/j.yexcr.2019.03.041
    • Daily, N. J., et al. (2017). High-Throughput Phenotyping of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes and Neurons Using Electric Field Stimulation and High-Speed Fluorescence Imaging. ASSAY and Drug Development Technologies. 15(4): 178-188. https://doi.org/10.1089/adt.2017.781
    • Daily, N. J., Santos, R., Vecchi, J., Kemanli, P., & Wakatsuki, T. (2017). Calcium transient assays for compound screening with human iPSC-derived cardiomyocytes: Evaluating new tools. Journal of evolving stem cell research, 1(2), 1.
    • Daily, N. J., et al. (2015). Journal of Bioengineering & Biomedical Science, 2015.

MTA Agreement

Material Transfer Agreement

IMPORTANT! PLEASE READ CAREFULLY BEFORE SUBMITTING AN ORDER. THIS IS A CONTRACT.

This Material Transfer Agreement (“MTA”) is between you (“Purchaser”) and the Applied StemCell, Inc. a California company, having its principal place of business at 521 Cottonwood Drive Suite 111, Milpitas, CA 95035 USA (“ASC”). Purchaser must have an approved, current ASC account to place an order. This MTA is effective for a period of five (5) years as of the last date of execution by the parties and governs the purchase and use of all ASC Materials under the terms and conditions set forth below.

TERMS AND CONDITIONS

Definitions

“ASC Material(s)” means materials acquired from ASC as documented on an ASC Sales Order, such as iPS Cell lines.

"ASC Sales Order" means an order submitted for ASC Materials in a form and format as determined by ASC from time to time. "Biological Material(s)" means ASC Materials, Progeny, Unmodified Derivatives and Modifications, either individually or jointly. "Commercial Use" means the sale, license, lease, export, transfer or other distribution of the Biological Materials to a person or entity not party to this MTA for financial gain or other commercial purposes and/or the use of the Biological Material: (a) to provide a service to a person or entity not party to this MTA for financial gain; (b) to produce or manufacture products for general sale or products for use in the manufacture of products ultimately intended for general sale (c) in connection with ADME (Absorption, Distribution, Metabolism and Excretion) testing; (d) in connection with drug potency or toxicity testing (e) in connection with proficiency testing service(s), including but not limited to, providing the service of determining laboratory performance by means of comparing and evaluating calibrations or tests on the same or similar items or materials in accordance with predetermined conditions; or (f) for research conducted under an agreement wherein a for-profit entity receives a right whether actual or contingent to the results of the research. Commercial Use specifically does not include Industry Sponsored Academic Research.

“Contributor(s)” means an organization(s) and/or individual(s) providing original material to ASC for deposit.

"Industry Sponsored Academic Research" means research sponsored by a for-profit organization carried out at a non-profit organization and by the non-profit organization’s employees. "Investigator" means the Purchaser’s principal scientist or researcher using the Biological Material(s). "Modification(s)" mean substances created by Purchaser which contain and/or incorporate a significant or substantial portion of ASC Material. "Progeny" means an unmodified descendant from the ASC Materials, such as virus from virus, cell from cell, or organism from organism. "Purchaser(s)" means the organization purchasing and receiving ASC Material pursuant to this MTA. "Unmodified Derivative(s)" mean substances created by Purchaser that constitute an unmodified functional sub-unit or product not changed in form or character and expressed by the ASC Material provided by ASC. Unmodified Derivatives include, but are not limited to, subclones of unmodified cell lines, purified or fractionated subsets of materials provided by ASC, proteins expressed by DNA/RNA supplied by ASC, or monoclonal antibodies secreted by a hybridoma cell line.

Scope of Use

Subject to the terms of this MTA, Purchaser’s Investigator may make and use the Biological Materials provided to Purchaser by ASC for research purposes only in Purchaser’s Investigator’s laboratory only. The Biological Materials are not intended for use in humans. Purchaser agrees that Biological Materials designated as biosafety level 2 or 3 constitute known pathogens and that other Biological Materials not so designated may be pathogenic under certain conditions. Purchaser assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, transfer and Purchaser’s use of the Biological Materials including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. Purchaser agrees that any activity undertaken with the Biological Materials will be conducted in compliance with all applicable guidelines, laws and regulations, and that Purchaser will obtain all permits, licenses or other approvals required by any governmental authority in connection with purchaser’s receipt, handling, storage, disposal, transfer and use of the Biological Materials.

Purchaser shall not distribute, sell, lend or otherwise transfer to a person or entity not party to this MTA the Biological Material, as defined above, for any reason, without ASC’s prior written agreement.

Any Commercial Use of the Biological Material is strictly prohibited without ASC’s prior written consent. Purchaser acknowledges and agrees that Purchaser’s use of certain Biological Material may require a license from a person or entity not party to this MTA, or be subject to restrictions that may be imposed by a person or entity not party to this MTA (“Third Party Terms”). To the extent of ASC’s knowledge of the existence of any such applicable rights or restrictions, ASC will take reasonable steps to identify the same, either in ASC’s catalog of ASC Materials and/or through ASC’s customer service representatives, and to the extent they are in the possession of ASC, ASC shall make information regarding such Third Party Terms reasonably available for review by Purchaser upon request. Purchaser expressly acknowledges that if there is a conflict between this MTA and the Third Party Terms, the Third Party Terms shall govern. Use of the Biological Materials may be subject to the intellectual property rights of a person or entity not party to this MTA, the existence of which rights may or may not be identified in the ASC catalog or website, and ASC makes no representation or warranty regarding the existence or the validity of such rights. Purchaser shall have the sole responsibility for obtaining any intellectual property licenses necessitated by its possession and use of the Biological Materials.

The use permitted under this MTA for Industry Sponsored Academic Research extends only to the academic research carried out at the non-profit organization and the non-profit organization’s employees. Any non-profit Purchaser using the Biological Materials in connection with Industry Sponsored Academic Research agrees to notify the industrial sponsor that any use of the Biological Materials by the industry sponsor will require a separate license from ASC and/or its Contributors and that ASC and/or its Contributors are under no obligation hereunder to license any Biological Materials to any such industry sponsor.

Warranty; Warranty Disclaimer

ASC warrants that (a) cells and microorganisms included in the ASC Material shall be viable upon initiation of culture for a period of thirty (30) days after shipment thereof from ASC and (b) any ASC Material other than cells and microorganisms shall meet the specifications on the applicable ASC Material product information sheet, certificate of analysis, and/or catalog description until the expiration date on the applicable ASC Material’s product label (such thirty (30) day period, or period until the expiration date, referred to herein as the “Warranty Period”). Purchaser’s exclusive remedy, and ASC’s sole liability, for breach of the warranties set forth in this paragraph is for ASC to, at ASC’s sole option, either (i) refund the fee paid to ASC for such ASC Material (exclusive of shipping and handling charges), or (ii) replace the ASC Material. The warranties set forth in this paragraph apply only if Purchaser handles and stores the ASC Material as described in the applicable ASC Material product information sheet. To obtain the exclusive remedy, Purchaser must report the lack of viability or non-conformation to specifications to ASC’s Technical Service Department within the applicable Warranty Period. Any expiration date specified on the ASC Material shipment documentation states the expected remaining useful life, but does not constitute a warranty or extend any applicable Warranty Period. Except as expressly provided above, the ASC Material and any technical information and assistance provided by ASC are provided as-is, without warranties of any kind, express or implied, including but not limited to any implied warranties of merchantability, fitness for a particular purpose, typicality, safety, accuracy and/or non-infringement.

Purchaser acknowledges that the ASC Material and any technical information and assistance provided by ASC are developed and provided exclusively for research purposes, and Purchaser agrees to use the same at its sole risk. Purchaser agrees that ASC and its Contributors will not be liable for any loss, claim or demand made by Purchaser, or made against Purchaser by any other party, due to or arising from the use of the ASC Material by Purchaser, except to the extent permitted by law when caused by the gross negligence or willful misconduct of ASC.

Limitation of Liability

In no event shall ASC or its Contributors be liable for any use of the Biological Material by Purchaser, for any loss, claim, damage, or liability, of whatever kind or nature, which may arise from or in connection with this MTA or the use, handling, storage, or disposal of the Biological Material. ASC’s liability to Purchaser for any claim related to or arising from this MTA or the Biological Material, whether in contract, warranty, tort, or otherwise, shall be limited to the amount paid by Purchaser for the Biological Material. In no event shall ASC be liable for any indirect, special, incidental, consequential, or punitive damages, including without limitation, loss of profits or loss of use, even if ASC has been advised of the possibility of such damages. The limitations of liability set forth in this MTA shall survive termination or expiration of this MTA for any reason.