ASE-9708

iPSC-Derived NK kit (AA)

Applied StemCell has developed an efficient integration-free method to differentiate high-quality natural killer cells (NK cells) from human iPSCs. The differentiated NK cells recapitulate the phenotype and functional parameters of primary and in vivo NK cells. We provide NK cells differentiated from an integration-free, control human iPSC line (ASE-9211), reprogrammed from fibroblasts of an African-American male donor. These cryopreserved, highpurity (≥ 80%) cells express high levels of NK cell biomarkers, CD56 and CD45 (Figure 1). To harness the full potential of our NK cells, we also provide optimized NK Culture Media (ASE9708MM) that supports the robust maintenance and functionality of the NK cells in culture. These iPSC-differentiated NK cells can be used as control lines to compare phenotype and functionality of patient-derived, genome edited iPSC-derived NK cells for drug screening applications. These NK cells can also be used as effector cells for cytotoxicity assays.