A 2A-CreERT2-WPRE-pA coexpression cassette is inserted before the stop codon of mouse Cd2 gene via homologous recombination to establish a tamoxifen inducible Cd2-CreERT2 tool stain. This strain can be used to knockout target gene in T cells and B cells after tamoxifen treatment when crossed with mice which carry loxp sites flanking target gene.
Figure 1. The result shows that expression pattern of RFP in T cells of Cd2-(2A-CreERT2-WPRE-pA); RFP.
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