The endogenous mice Fas gene was replaced by human FAS gene.
Figure 1. Detection of FAS expression in thymus and lung by RT-PCR.Â
Wild type: only one band at 286 bp with primers F1/R1 (mFas);Heterozygous: one band at 286 bp with primers F1/R1 (mFas) and one band at 304 bp with primers F2/R2 (hFAS); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.
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