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CD3EDG (BALB/c)

Name: CD3EDG (BALB/c)
SKU: ASHU-220122
Availability: OutOfStock

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SKU: ASHU-220122

Category: Uncategorized

The endogenous mouse Cd3e/Cd3d/Cd3g genes were replaced by human CD3E/CD3D/CD3G gene.

Validation Data

Figure 1. Detection of surface expression of human CD3E/CD3D on T cells in spleen in homozygous hCD3EDG (BALB/c) mice. (Data in partnership with collaborators)

landingpage-ashu-220122-CD3EDG-hu-icpmouse-2

Figure 2. Detection of surface expression of human CD3E/CD3G on T cells in blood in homozygous hCD3EDG (BALB/c) mice. (Data in partnership with collaborators)

landingpage-ashu-220122-CD3EDG-hu-icpmouse-3

Figure 3. Analysis of TCR signalling in hCD3EDG (BALB/c) mice upon CD3/CD28 activation in vitro. (Data in partnership with collaborators)

Note: CFSE-dilution as a measure of mouse T cell proliferation.

CD3+ T cells were isolated in the splenocytes from WT BALB/c and hCD3EDG mice. Isolated T cells were labeled with µCFSE dye and stimulated with anti-mCD3 (5µg/mL) or anti-hCD3 (5µg/mL) plus soluble anti-mCD28 (50µg/mL) for in vitro culture for 72 hr. T cell proliferation was analyzed by flow cytometry for CFSE dilution.

Size	Mouse

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Material Transfer Agreement

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This Material Transfer Agreement (“MTA”) is between you (“Purchaser”) and the Applied StemCell, Inc. a California company, having its principal place of business at 521 Cottonwood Drive Suite 111, Milpitas, CA 95035 USA (“ASC”). Purchaser must have an approved, current ASC account to place an order. This MTA is effective for a period of five (5) years as of the last date of execution by the parties and governs the purchase and use of all ASC Materials under the terms and conditions set forth below.

TERMS AND CONDITIONS

Definitions

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CD3EDG (BALB/c)

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Validation Data

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CTLA4 & CD28 (BALB/c)

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CD3EDG (BALB/c)

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Validation Data

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MTA Agreement

Material Transfer Agreement

TERMS AND CONDITIONS

Definitions

Scope of Use

Warranty; Warranty Disclaimer

Limitation of Liability