| Table Header | Table Header |
|---|---|
|
Catalog Number |
ASHU-220122 |
|
Model Name |
CD3EDG (BALB/c) |
|
Strain Name |
BALB/c-Cd3etm1(hCD3E)Cd3dtm1(hCD3D)Cd3gtm1(hCD3G)/Asc |
|
GENE Synonyms |
CD3, T3e, AI504783, CD3epsilon; T3d; T3g, Ctg3, Ctg-3 |
|
Human Orthologs |
CD3E; CD3D; CD3G |
|
Ensembl ID |
ENSMUSG00000032093; ENSMUSG00000032094; ENSMUSG00000002033 |
|
MGI ID |
88332; 88331; 88333 |
|
NCBI ID |
12501; 12500; 12502 |
|
Background |
BALB/c |
|
Coat Color |
White |
|
Model Type |
Humanized |
|
Model Color |
White |
The endogenous mouse Cd3e/Cd3d/Cd3g genes were replaced by human CD3E/CD3D/CD3G gene.
Figure 1. Detection of surface expression of human CD3E/CD3D on T cells in spleen in homozygous hCD3EDG (BALB/c) mice. (Data in partnership with collaborators)
Figure 2. Detection of surface expression of human CD3E/CD3G on T cells in blood in homozygous hCD3EDG (BALB/c) mice. (Data in partnership with collaborators)
Figure 3. Analysis of TCR signalling in hCD3EDG (BALB/c) mice upon CD3/CD28 activation in vitro. (Data in partnership with collaborators)
Note: CFSE-dilution as a measure of mouse T cell proliferation.
CD3+ T cells were isolated in the splenocytes from WT BALB/c and hCD3EDG mice. Isolated T cells were labeled with µCFSE dye and stimulated with anti-mCD3 (5µg/mL) or anti-hCD3 (5µg/mL) plus soluble anti-mCD28 (50µg/mL) for in vitro culture for 72 hr. T cell proliferation was analyzed by flow cytometry for CFSE dilution.
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