ASE-9742

iPSC-Derived Human Dopaminergic Neuron Kit (African-American, Male Line) kit

Applied StemCell has developed an efficient integration-free, small molecule-based method to differentiate high-quality dopaminergic (DOPA) neurons from human iPSCs. The differentiated DOPA neurons recapitulate the phenotype and functional parameters of primary and in vivo dopaminergic neurons. We provide dopaminergic neurons differentiated from an integration-free, control human iPSC line (ASE-9211), reprogrammed from the fibroblasts of an African-American male donor. These high purity (≥70%) cells express high levels of the dopaminergic neuron biomarker TH (Figure 1). To harness the full potential of our DOPA neurons, we also provide optimized DOPA Neuron Basal Media (ASE-9742MM) and DOPA Neuron Supplement (ASE-9742MM-A) that support robust maintenance and functionality of the dopaminergic neurons in culture. These iPSC-differentiated DOPA neurons can be used as control lines to compare phenotype and functionality of patient-derived, genome edited iPSC-derived DOPA neurons for drug screening applications. These dopaminergic neurons can also be used as effector cells for cytotoxicity assays.