ASE-9215

Human Induced Pluripotent Stem Cells (from Normal, Male, Peripheral Blood Mononuclear Cells (PBMC))
iPSC (PBMC derived) 1ml – 1.0 x 10^6 cells MTA required, research use only

Applied StemCell, Inc. provides Control Human Induced Pluripotent Stem (iPS) cells at low passages (p15). These pluripotent cells were generated from normal peripheral blood mononuclear cells (PBMC) using a Sendai virus method. This method allows the expression of human transcription factors (OCT4, SOX-2, KLF4, and c-MYC) that initiate the reprogramming process. The resulting human iPS cells (hiPSCs) were selected using morphological criteria without the use of either fluorescent markers or drug selection. These iPS cells have been tested for expression of pluripotency markers (Oct-4, Sox-2, Nanog, and SSEA-4; Figure 2) and for normal male karyotype (Figure 3). The ASE-9215 control human iPSC line can be used for CRISPR/Cas9 genome editing and differentiation to somatic lineages in vitro. Detailed protocols for thawing, culturing under feeder-free conditions, and cryopreservation of these iPS cells are provided.