A V5-IRES-luci-2A-EGFP expression cassette was inserted before the stop codon TAA of mouse Cd8a gene to establish a multi-labeled knock-in model with V5, luciferase and EGFP fluorescent protein.

Vallidation Data

landingpage-aski-18030-Cd8a-IRES-Luc-2A-EGFP-ki-mouse-1

Figure 1.  In the absence of any stimulation, the in vivo imaging system detected that Cd8aV5-IRES-luci-2A-EGFP/+ mice showed significant fluorescence. 

landingpage-aski-18030-Cd8a-IRES-Luc-2A-EGFP-ki-mouse-2

Figure 2.  In vivo imaging validation of MC38 tumors in Cd8aV5-IRES-luci-2A-EGFP/+ reporter mice treated or not with a PD-1 mAb. After 1 day of stimulation with PD1 antibody, the fluorescence was significantly enhanced.

landingpage-ashki-18030-Cd8a-IRES-Luc-2A-EGFP-ki-mouse-3

Figure 3.  EGFP expression analysis in  PBMC of Cd8aV5-IRES-luci-2A-EGFP/+ mice by flow cytometry.

Size

Mouse

Reviews

There are no reviews yet.

Be the first to review “Cd8a-IRES-Luc-2A-EGFP”