iPSC-Derived Natural Killer (NK) Cells With Media

$1,980.00$3,080.00

SKU ASE-9285-1 Categories , ,

iPSC-Derived Natural Killer (NK) Cells

With Media

We use our efficient integration-free, small molecule-based method to differentiate high-quality natural killer (NK) cells from human iPSCs at passage 0 (P0). The differentiated NK cells recapitulate the phenotype and functional parameters of primary and in vivo NK cells.

We provide NK cells differentiated from an integration-free, control human iPSC line (ASE-9211), reprogrammed from fibroblasts of an African American male donor. These cryopreserved, high-purity (>80%) cells express high levels of NK biomarkers CD56 and CD45.

These iPSC-differentiated NK cells can be used as control lines to compare the phenotype and functionality of patient-derived, genome-edited iPSC-derived NK cells for drug screening applications. These NK cells can also be used as effector cells in cytotoxicity assays.

Figure 1. Flow cytometry analysis of ASE-9708 iPSC-derived NK cells for NK cell biomarkers. Cryopreserved NKs, differentiated from Applied StemCell’s control iPSC line, ASE-9211 were recovered in NK culture media. The cells were stained with NK cell markers, CD45 and CD56 at day 2. Left: Isotype control antibodies. Right: CD45/CD56.

Tumor cell killing activity

iPSC-derived NK cells (ASE-9285-1) display robust activity against hematopoietic tumor cell line K562. We mixed Calcein AM-stained K562 cells (target) with ASE-9285-1 NK cells (effector) at effector/target (E/T) ratios ranging from 0.625—5, incubated for 16 hours, and measured fluorescence.

Donor information

Table Header Table Header
Age
Neonate
Gender
Male
Ethnicity
African American
Tissue source
Dermal fibroblasts
Reprogramming method
Episomal
Culture conditions
Feeder-free

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