iPSC-derived P0 NK cell kits

20% off for a limited time!

Scale up screening and improve the consistency and interpretability of your results by working with P0 iPSC-derived NK cells instead of primary NK cells.
Our P0 iPSC-derived NK cells are available with two different backgrounds:

For general research
ASE-9285-1 cells are derived from ASE-9211, our control iPSC cell line reprogrammed from normal male fibroblasts using an episomal method.

Each vial contains ≥1 x 106 P0 cells/vial, ≥80% purity by flow cytometry
$1800/vial, now $1440/vial.

Visit the ASE-9285-1 product page to add to cart>

For general research and therapeutic discovery studies
ASE-9285-3
 cells are derived from ASE-9250, our iPSC cell line reprogrammed from normal male CD34+ umbilical cord blood cells using an episomal method.

Choose ASE-9285-3 if you anticipate needing matching GMP cells—ASE-9280 iPSCs are the GMP version of ASE-9250 iPSCs and can be differentiated into NK cells under GMP compliance.

Each vial contains ≥1 x 106 P0 cells/vial, ≥80% purity by flow cytometry
$2800/vial, now $2240/vial

Visit the ASE-9285-3 product page to add to cart>

NOTE: We also have a limited amount of primary NK cells in stock if you’d like to order primary cell controls for the iPSC-derived NK cells—ask us in the form to learn more.

Quality control assays
Each lot of ASE-9285-1 and ASE-9285-3 meets the following standards before release

  • ≥ 80% purity using flow cytometry and NK cell markers CD45 and CD56
  • Each lot tested growth, viability, and the absence of mycoplasma and pathogens

Kit components
Each kit contains cells and optimized media:

  • 1 vial of P0 iPSC-derived NK cells, ≥1 x 106 cells/ vial
  • 100 mL of NK Cell Basal Culture Media (for cytotoxicity assays only, at this time)
  • 1 mL of NK Cell Culture Media Supplement A (100x)

iPSC-derived NK cells show tumor cell killing activity

iPSC-derived NK cells (ASE9285-1) display robust activity against hematopoietic tumor cell line K562. We mixed Calcein AM-stained K562 cells (target) with ASE-9285-1 NK cells (effector) at effector/target (E/T) ratios ranging from 0.625—5, incubated for 16 hours, and measured fluorescence.

Each lot of iPSC-derived NK cells is ≥ 80% NK cells

Flow cytometry analysis of ASE-9285-1 iPSC-derived NK cells for NK cell biomarkers. Cryopreserved NK cells, differentiated from Applied StemCell’s control iPSC line, ASE-9211, were recovered in NK culture media. The cells were stained with NK cell markers, CD45 and CD56, at day 2.