Scale up screening and improve the consistency and interpretability of your results by working with P0 iPSC-derived NK cells instead of primary NK cells.
Our P0 iPSC-derived NK cells are available with two different backgrounds:
For general research
ASE-9285-1 cells are derived from ASE-9211, our control iPSC cell line reprogrammed from normal male fibroblasts using an episomal method.
Each vial contains ≥1 x 106 P0 cells/vial, ≥80% purity by flow cytometry$1800/vial, now $1440/vial.
Visit the ASE-9285-1 product page to add to cart>
For general research and therapeutic discovery studies
ASE-9285-3 cells are derived from ASE-9250, our iPSC cell line reprogrammed from normal male CD34+ umbilical cord blood cells using an episomal method.
Choose ASE-9285-3 if you anticipate needing matching GMP cells—ASE-9280 iPSCs are the GMP version of ASE-9250 iPSCs and can be differentiated into NK cells under GMP compliance.
Each vial contains ≥1 x 106 P0 cells/vial, ≥80% purity by flow cytometry$2800/vial, now $2240/vial
Visit the ASE-9285-3 product page to add to cart>
NOTE: We also have a limited amount of primary NK cells in stock if you’d like to order primary cell controls for the iPSC-derived NK cells—ask us in the form to learn more.
Quality control assays
Each lot of ASE-9285-1 and ASE-9285-3 meets the following standards before release
Kit components
Each kit contains cells and optimized media:
iPSC-derived NK cells (ASE9285-1) display robust activity against hematopoietic tumor cell line K562. We mixed Calcein AM-stained K562 cells (target) with ASE-9285-1 NK cells (effector) at effector/target (E/T) ratios ranging from 0.625—5, incubated for 16 hours, and measured fluorescence.
Flow cytometry analysis of ASE-9285-1 iPSC-derived NK cells for NK cell biomarkers. Cryopreserved NK cells, differentiated from Applied StemCell’s control iPSC line, ASE-9211, were recovered in NK culture media. The cells were stained with NK cell markers, CD45 and CD56, at day 2.