Humanized Gene Knockout Strategy
PCR Gel Electrophoresis to Genotype Recombinant F1 Mice
Figure 1. In WT and HE mice, the ability of P1/P2 primers to amplify large bands does not affect the interpretation of the results as the primers were designed to amplify KO bands.
WT: P1/P2: 11152bp; P3/P4: 779bp
HE: P1/P2: 11152bp and 1.6 kb; P3/P4: 779bp
HO: P1/P2: 1.6 kb; P3/ P4: no product
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