Ace2-KO

Humanized Gene Knockout Strategy

PCR Gel Electrophoresis to Genotype Recombinant F1 Mice

Figure 1. In WT and HE mice, the ability of P1/P2 primers to amplify large bands does not affect the interpretation of the results as the primers were designed to amplify KO bands.

WT: P1/P2: 11152bp; P3/P4: 779bp

HE: P1/P2: 11152bp and 1.6 kb; P3/P4: 779bp

HO: P1/P2: 1.6 kb; P3/ P4: no product