PCR Gel Electrophoresis to Genotype Recombinant F1 Mice
Figure 1. In WT and HE mice, the ability of P1/P2 primers to amplify large bands does not affect the interpretation of the results as the primers were designed to amplify KO bands.
WT: P1/P2: 11152bp; P3/P4: 779bp
HE: P1/P2: 11152bp and 1.6 kb; P3/P4: 779bp
HO: P1/P2: 1.6 kb; P3/ P4: no product
Ready to learn more?
Fill out the contact form below and a team member will be in touch within one business day.
We use cookies to ensure that we give you the best experience on our website. If you continue to use this site we will assume that you are happy with it.Ok