Strain Information

Table Header Table Header
Catalog Number
ASHU-190013
Model Name
APOE2
Strain Name
C57BL/6J – Apoetm4(hAPOE2)ASC
GENE Synonyms
Apo-E; AI255918
Human Orthologs
APOE
Ensembl ID
ENSMUSG00000002985
MGI ID
88057
NCBI ID
11816
Background
C57BL/6J
Coat Color
Black
Mutation Type
Humanized
Model Type
Humanized
Research Application
Immunotherapy
Model Color
Black

Humanized Strategy

Landingpage-ASHU-190013-APOE2-HU-ICPMouse-1


Validation Data

Landingpage-ASHU-190013-APOE2-HU-ICPMouse-2

Figure 1. Genotyping of WT, KO &  Humanized mice by PCR. Primer P1/2 Wild type = 342 bp Primer P3/4 mutant = 332 bp M: 100bp Plus DNA ladder.

Landingpage-ASHU-190013-APOE2-HU-ICPMouse-3

Figure 2. Western blot analysis of  APOE2  protein from WT, KO & Humanized mice.  APOE anti-body: Sc-13521 (santa cruz)

Validate Human APOE Expression by Western Blot

Landingpage-ASHU-190013-APOE2-HU-ICPMouse-4

KO: Apoe knockout mouse sample; hAPOE2 : humanized APOE2 mouse sample (ASHU-190013); hAPOE3 : humanized APOE3 mouse sample (ASHU-190003); hAPOE4 : humanized APOE4 mouse sample (ASHU-190002); WT: wild type mouse sample; M: marker.

Ready to learn more?

Fill out the contact form below and a team member will be in touch within one business day.