We provide Control Human Induced Pluripotent Stem (iPS) cells at low passages (p16). These pluripotent cells were generated from normal human skin fibroblasts using episomal reprogramming methods. These methods allow the transient expression of human transcription factors (OCT4, SOX2, KLF4, and c-MYC) that initiate the reprogramming process. The resulting human iPS cells (hiPSCs) were selected using morphological criteria without the use of either fluorescent markers or drug selection.
These iPS cells have been tested for the expression of the pluripotency markers OCT4, SOX2, SSEA4, TRA-1-60, and TRA-1-81, and for normal female karyotype.
The ASE-9209 control human iPSC line can be used for CRISPR/Cas9 genome editing and differentiation to somatic lineages in vitro.
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