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Human iPSCs From Fibroblasts (Normal, Male, African American, Neonate, NIST Line)

Human iPSCs From Fibroblasts (Normal, Male, African American, Neonate, NIST Line)

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SKU ASE-9211 Categories , ,

Human iPSCs from Fibroblasts

Normal, Male, African American, Neonatal

Start your research with high quality human induced pluripotent stem cells (hiPSCs)

  • Ready-to-use iPSCs from Applied StemCell accelerate your studies with quality reagents
  • Episomal reprogramming and morphology-based selection preserves purity by reducing sources of heterogeneity
  • Reprogrammed from fibroblasts from a male, African American donor
  • Pluripotency confirmed through OCT4, SOX2, SSEA4, TRA-1-60, and TRA-1-81, and alkaline phosphatase (AP)

Overview

Applied StemCell offers high-quality hiPSCs (ASE-9211), generated from normal human skin fibroblasts using episomal reprogramming methods. This transient reprogramming approach utilizes human transcription factors (OCT4, SOX2, KLF4, and c-MYC) to initiate the reprogramming process without integrating foreign DNA into the genome. The iPSCs are selected based on precise morphological criteria, eliminating the need for fluorescent markers or drug-based selection, ensuring their purity and natural state.   Each order of ASE-9211 contains one vial of 0.5 x 106 cells. 

How it works

The ASE–9211 cell line is user-friendly, offering a seamless blend of safety, flexibility, and reliability.

1. Thaw and expand ASE-9211. 
  • Prepare Matrigel® or Geltrex-coated plates and thaw mTeSR-plus medium with Rock Inhibitor and Primocin.
  • Plate the cells into prepared wells and evenly distribute clumps for uniform growth.
  • Monitor colonies for attachment and stability; change the medium as needed.
2. Passaging human iPSCs 
  • Detach cells using a 0.5 mM EDTA solution.  
  • Replate cells into freshly coated wells, ensuring colonies remain intact. 
  • Incubate under appropriate conditions. 
3. Cryopreserving human iPSCs 
  • Detach colonies with EDTA and resuspend them in freezing medium. 
  • Transfer the suspension to labeled cryovials and store at -80°C overnight in a CoolCell® Freezing Container. Move cryovials to liquid nitrogen the next day for long-term storage. 

Can’t find the right iPSCs for your research? We offer custom reprogramming services to meet your specific needs.

ASE-9211 shows all pluripotency markers

Figure 1: We performed immunostaining with pluripotency markers OCT-4, SOX2, SSEA-4, TRA-1-60 and TRA-1-81(Top Row) and DAPI (Bottom row).

ASE-9211 can differentiate into 3 lineages

Figure 2: We performed immunostaining of hiPSC to confirm lineage commitment using nuclear marker DAPI (blue) Mesoderm (ME) marker: GATA4 (green); Ectoderm (EC) marker: β-Tubulin (red).

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Frequently asked questions

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Yes, our ASE–9211 iPSC line is optimized for CRISPR/Cas9 editing, making it ideal for genetic studies and functional genomics.

We provide detailed protocols for thawing, feeder-free culturing, and cryopreservation, ensuring ease of use for your research team.

Each batch is tested for pluripotency markers (OCT4, SOX2, SSEA4, TRA-1-60, TRA-1-81) and alkaline phosphatase (AP) activity, alongside verification of a normal male karyotype to ensure purity and stability.

Each vial of the product is at P17 with 0.5 million cells / vial.

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