ASE-9216

Human Induced Pluripotent Stem Cells (from Normal, Male, Human Dermal Fibroblasts (HDF))
iPS Cells (iPSCs from HDF, Caucasian, Male, Neonatal) 1.0 x 10^6 cells/vial MTA required

Applied StemCell, Inc. provides Control Human Induced Pluripotent Stem (iPS) cells at low passages (p8). These pluripotent cells were generated from normal human dermal fibroblasts (HDF) using a Sendai virus reprogramming method. This method allows the transient expression of human transcription factors (OCT4, SOX-2, KLF4, and c-MYC) that initiate the reprogramming process. The resulting human iPS cells (hiPSCs) were selected using morphological criteria without the use of either fluorescent markers or drug selection. These iPS cells have been tested for normal male karyotype (Figure 1 and 2) and pluripotency (Figure 3 and 4). The ASE-9216 control human iPSC line can be used for CRISPR/Cas9 genome editing and differentiation to somatic lineages in vitro. Detailed protocols for thawing, culturing under feeder-free conditions, and cryopreservation of these iPS cells are provided.