Figure 1: Agarose gel electrophoresis for in vitro transcribed Cas9 mRNA and guideRNA.
Figure 2. For genotyping, PCR followed by Sanger Sequencing was used to identify homozygous, heterozygous and wild type mice.
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This Material Transfer Agreement (“MTA”) is between you (“Purchaser”) and the Applied StemCell, Inc. a California company, having its principal place of business at 521 Cottonwood Drive Suite 111, Milpitas, CA 95035 USA (“ASC”). Purchaser must have an approved, current ASC account to place an order. This MTA is effective for a period of five (5) years as of the last date of execution by the parties and governs the purchase and use of all ASC Materials under the terms and conditions set forth below.
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