H11-(CAG-LSL-mTagBFP2)

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SKU ASKI-190006 Category Tags ,

Description

A CAG-LSL-mTagBFP2-3xFLAG-WPRE-polyA cassette was inserted at the mouse H11 locus using CRISPR gene editing technology. When bred to a strain expressing Cre recombinase under control of various tissue specific promoters, Cre recombination deletes the LSL cassette and allows the expression of the mTagBFP2-3xFLAG protein.

Validation Data

landingpage-aski-190006-H11-CAG-LSL-mtagBFP2-ki-mouse-1

Figure 1. BFP expression in CAG-mTagBFP2/+ mouse, visualized using fluorescence imaging.

CAG-mTagBFP2/+ mouse was achieved through crossing CAG-LSL-mTagBFP2/+ mouse and Cre-expressing mouse. BFP expression in different organs of CAG-mTagBFP2/+ mouse was detected using fluorescence imaging, which suggests relatively high expression of BFP in a variety of organs. A: Images of different organs of CAG-mTagBFP2/+ mouse; B: Images of histologic sections of CAG-mTagBFP2/+ mouse under confocal microscopy.