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ActiCells™ GMP-Matching RUO hiPSCs (CD34<sup>+</sup> Cord Blood Cells, Normal, Male)

ActiCells™ GMP-Matching RUO hiPSCs (CD34+ Cord Blood Cells, Normal, Male)

SKU ASE-9250 Categories , , ,

ActiCells™ GMP-Matching RUO hiPSCs

CD34+ Cord Blood Cells, Normal, Male

Move seamlessly from research to GMP-compliant production.
  • Favorable development profile–reprogrammed from CD34+ cord blood cells for reduced immunogenicity and fewer somatic mutations compared to reprogrammed adult cells
  • Efficient–isogenic with ActiCells™ GMP hiPSCs (Cat. # AST-9280) for streamlined transition to GMP manufacturing
  • Transgene-free–episomally reprogrammed
  • Well-characterized–assessed for pluripotency markers, genetic purity, and post-cryopreservation viability
  • Gene-editing ready–suitable for cell line engineering using CRISPR, Mad7, and TARGATT™ Large Knock-in Technology
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Overview

Reprogrammed from CD34+ cord blood cells, ActiCells™ GMP-Matching RUO hiPSCs (Cat # ASE-9250) are an efficient, high-quality starting point for your cell-based therapy development. The reduced immunogenicity and lower somatic mutation load compared to adult cells give iPSCs reprogrammed from CD34+ cord blood cells a favorable therapeutic development profile, and the proven quality of iPSCs generated by Applied StemCell make ActiCells™ GMP-Matching RUO hiPSCs the reliable choice for your therapeutic development program.

As the isogenic RUO version of our ActiCells™ GMP hiPSCs, ActiCells™ RUO hiPSCs are an efficient and cost-effective way to optimize your cell-based therapy before moving into GMP manufacturing. Our team can also provide GMP-compliant iPSC gene editing, differentiation, cell banking, and manufacturing services to further streamline your path to the clinic.

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Each vial of ActiCells™ GMP-Matching RUO hiPSCs contains 1 x 106 cells. Every lot is tested for viability following recovery from cryopreservation, functional pluripotency (formation of three germ layers), expression of pluripotency markers (Oct4, Sox2, Nanog, SSEA-4, and Tra-1-60), presence of alkaline phosphatase (AP), STR, sterility, and for the absence of mycoplasma and pathogens (CofA available upon request).
Table Header Table Header
Reprogramming method
Episomal
Donor tissue
CD34+ Umbilical Cord Blood Cells
Gender
Male
Clinical information
Normal
Quantity
1 x 106 cells/vial

Supporting data

ActiCells™ GMP-Matching RUO hiPSCs maintain pluripotent stem cell morphology after thawing

Figure 1. ActiCells™ GMP-Matching RUO hiPSCs maintain pluripotent stem cell morphology 24 hours (left) and 72 hours (right) after thawing.

ActiCells™ GMP-Matching RUO hiPSCs are pluripotent

Figure 2. ActiCells™ GMP-Matching RUO hiPSCs show strong, uniform expression of pluripotency markers Oct4, Sox2, Nanog, SSEA-4, and TRA-1-60 (top row) with DAPI staining (blue) on the bottom row.

Figure 3. ActiCells™ GMP-Matching RUO hiPSCs differentiate into the three germ layers, demonstrating excellent differentiation potential. (A) Endoderm is demonstrated by alpha-fetoprotein (AFP) immunofluorescence (IF, green), (B) ectoderm by Neuron-specific class III β-tubulin (Tuj1) IF, and (C) mesoderm by smooth muscle actin (SMA) IF. DAPI staining in blue.

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Frequently asked questions

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ActiCells™ GMP-Matching RUO hiPSCs are intended for research use only. They are isogenic with ActiCells™ GMP hiPSCs (ASE-9280) and are typically used for optimization before moving into the matching GMP line. 

Each vial contains 1 x 106 cells, shipped on dry ice. Upon receipt, store in a liquid nitrogen freezer. For optimal results, use within six months of receipt.

Yes, a certificate of analysis (CofA) is available upon request, providing full details on viability, sterility, short tandem repeat profiling (STR), and mycoplasma testing.

We provide detailed protocols for thawing, feeder-free culturing, and cryopreservation, ensuring ease of use for your research team.

ASE-9250 iPSCs are derived from CD34+ umbilical cord blood cells using episomal reprogramming.

Episomal reprogramming is a non-integrative method, meaning it does not alter the genome, reducing the risk of genetic mutations.

Yes, ActiCells™ GMP-Matching RUO hiPSCs are validated for key pluripotency markers (Oct4, Sox2, Nanog, SSEA-4, TRA-1-60) and undergo rigorous quality control for genetic purity and viability.

Yes, ActiCells™ GMP-Matching RUO hiPSCs are an ideal starting point for gene-editing applications, including CRISPR/Cas9 modifications.

ActiCells™ GMP-Matching RUO hiPSCs can differentiate into all three germ layers (endoderm, mesoderm, ectoderm), making them suitable for diverse research applications.

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