iPS Cells Research Grade (iPSCs from CD34+ Cord Blood, Male)

SKU ASE-9250 Categories GMP Grade iPSC Services & Products, GMP iPSC, Human Induced Pluripotent Stem Cells (hiPSCs), Normal Tag Service

Description

Applied StemCell, Inc. provides Human Induced Pluripotent Stem (hiPS) Cells (Research Grade) that were generated from CD34+ umbilical cord blood cells using an epsiomal reprogramming method. The ASE-9250 research grade hiPSC line is from a male donor and has been fully characterized. This includes expression of pluripotency markers (Oct4, Sox2, Tra-1-60, and Tra-1-81; Figure 2), alkaline phosphatase (AP) staining (Figure 3), STR analysis (Figure 5), and the ability to differentiate into the three germ layers (Figure 4). The ASE-9250 human iPSC line can be used for CRISPR/Cas9 genome editing and differentiation to somatic lineages in vitro.

Reprogramming Method	Episomal
Tissue	CD34+ Umbilical Cord Blood Cells
Sex	Male
Clinical Information	Normal
Quantity	1 x 10⁶ cells/vial
Shipping	Dry ice
Storage and Stability	Store in liquid nitrogen freezer immediately upon receipt. This product is stable for at least 6 months from the date of receiving when stored as directed.
Quality Control	Each lot of human iPS cells has been tested for viability following recovery from cryopreservation. In addition, the hiPSCs have also been tested for functional pluripotency (formation of the three germ layers), expression of pluripotency markers (Oct4, Sox2, Tra-1-60, and Tra-1-81), presence of alkaline phosphatase (AP), STR, sterility, and for the absence of mycoplasma and pathogens (CofA available upon request).