PD-1 & PD-L1

Strategy for Generating Humanized PD-1 Mouse Model

Strategy for Generating Humanized PD-L1 Mouse Model

Expression of hPD-1 and hPD-L1 was Confirmed by Flow Cytometry in Double-humanized PD-1 and PD-L1 Mice

Figure 1. The expression of human PD-1 and PD-L1 in double humanized PD-1&PD-L1 mice was confirmed by FACS.

In Vivo Validation of the Double Humanized PD-1 and PD-L1 Mice Demonstrate Their Suitability for Antibody Efficacy Studies

Figure 2. In vivo validation of double humanized PD-1&PD-L1 mice. The double humanized mice were inoculated with MC38 cells, and randomly assigned to different groups (n=8) when the tumor grew to a volume of 100 mm3. A combinatorial treatment of anti-PD-L1 and anti-PD-1 demonstrated a noticeable improvement in efficacy of the drugs compared to the same dose of single agent (top figure) without affecting the body weight of the animals (bottom figure).