Insert confidently with our TARGATT™ Knock-in Kit:
The TARGATT™ HEK293 High-Efficiency Sub-Line (HESL) Knock-in Kit (H11) for Library Screening (FACS) is designed for fast, efficient, and site-specific integration of DNA fragments of up to 20 kb into HEK293 cells. The master cell line provided in this kit contains the TARGATT™ landing pad with an attP site engineered into the H11 safe harbor locus. The kit also contains a cloning plasmid containing a corresponding “attB” sequence into which any gene of interest can be cloned. The expression of the integrase (accomplished via the provided plasmid) mediates stable integration of the transgene into the master cell line (Figure 1). The TARGATT™ integrase enables highly efficient (>60% without enrichment, >90% with enrichment), and site-specific DNA integration without disruption of internal genes.
The TARGATT™ HEK293 High-Efficiency Sub-Line (HESL) Knock-in Kit (H11) for Library Screening (FACS) is ideal for single-transgene-per-cell knock-in and uniform, stable expression of your protein.
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