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TARGATT™ HEK293 Knock-in Kit (H11)

TARGATT™ HEK293 Knock-in Kit (H11)

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TARGATT™ HEK293 Knock-in Kit (H11)

Insert confidently with our TARGATT™ Knock-in Kit:

  • High efficiency integration
  • Site-specific, stable knock-in cell line generation
  • Single copy gene integration into safe harbor locus
  • Gene expression from an active, intergenic locus
  • Low off-target integration

Overview

The TARGATT™ HEK293 Knock-in Kit (H11) is designed for fast and site-specific knock-in in HEK293 cells, using an easy-to-use gene knock-in approach. The master cell line provided in this kit contains an “attP” integrase recognition landing pad engineered into the hH11 safe harbor locus in the genome. The kit also contains a cloning plasmid containing a corresponding “attB” sequence into which any gene of interest can be cloned (under control of the CMV promoter or a promoter-of-choice). The expression of the integrase (provided as an integrase plasmid) mediates the stable integration of the transgene into the master cell line (Figure 1). The TARGATT™ integrase technology enables highly efficient (>40% without enrichment and ~90% with enrichment), and site-specific DNA integration without disruption of internal genes.

The TARGATT™ HEK293 Knock-in Kit (H11) is ideal for single transgene gene knock-in and uniform, stable expression of your protein. If you’re building mammalian cell libraries, contact us to learn about our TARGATT™ library cloning plasmids.

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Our knock-in strategy

Figure 1. Schematic representation of TARGATT™ site-specific transgene integration mediated by integrase. The TARGATT™ HEK Master Cell Line was engineered with the attP landing pad at the hH11 safe harbor locus. The TARGATT™ plasmid containing the integrase recognition site, attB is used to clone the transgene. The integrase catalyzes an irreversible reaction between the attP site in the genome and attB site in the donor vector, resulting in integration of the gene of interest at the selected H11 locus. The cells containing the gene of interest can be enriched using the selection marker (gray box).

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