Tgtn (CAG-human ACE2-IRES-Luciferase)

Strategy of TgTn (CAG-human ACE2-IRES-Luciferase-WPRE-polyA) Transgenic Mouse Model

Figure 1: Construction strategy of TgTn (CAG-human ACE2-IRES-Luciferase-WPRE-polyA) transgenic mouse model

Plasmid Map

Figure 2: Plasmid map of the TgTn (CAG-human ACE2-IRES-Luciferase-WPRE-polyA) transgenic mouse model targeting vector

Vector Evaluated by Restriction Endonucleases

Figure 3: Agarose gel electrophoresis of the vector digested by EcoRI. Lane 1 represents three fragments of length 7805bp, 2703 bp and 415bp in the EcoRI digest. M: 1 kb DNA ladder (from Fermentas).

PCR Genotyping Result of Positive F0 Generation

Figure 4: PCR genotyping result of positive F0 generation. A. Genotyping Strategy of F0 mice: TG=819 bp, WT=0 bp. B. Agarose gel electrophoresis of PCR products: Ten positive F0 generation mice were obtained (Nos.: 17, 18, 21, 22, 23, 30, 35, 38, 40, 47). Abbreviations: Nos.: positive F0 mouse ID numbers; TG: Transgenic; WT: wide type; M: 1kb DNA ladder from Fermentas）

PCR Genotyping Method for Identifying F0 Generation

Luciferase Expression in Transgenic Mouse Model

Figure 5: Detection of luciferase expression in C57BL/6J-TgTn(CAG-human ACE2-IRES-Luciferase-WPRE-polyA) mice. The images shown are representative images are from the abdomen (A) and back (B). Mice with ID No. 17, 21, 22, 30, 35, 38, 40, 47 showed strong fluorescence. The color scale in intensity/sec is shown on the right.