Teratoma Formation

A gold standard for establishing the pluripotency of iPSCs and ESCs is their ability to form cells of the three germ layers (endoderm, mesoderm and ectoderm). Teratoma are formed when pluripotent stem cells are injected into mice and spontaneously differentiate into cells of the three germ layers. Teratoma formation is considered as a standard index of iPSC or ESC. Applied StemCell has offered this service for over 15 years and have enabled researchers to characterize their pluripotent stem cells effectively as evidenced by our publications. We can do the teratoma assay for your iPSCs and ESCs so that you can move ahead with the critical aspects of your research.

For clinical iPSC derived products, it is critical that the differentiated cells are pure and free from undifferentiated iPSC contamination. Teratoma formation analysis is the method to test the purity and safety in vivo for iPSC differentiated cell products.