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ASC’s cGMP Grade iPSC Line
Applied StemCell’s cGMP Grade iPSC Line was generated from male, CD34+ cord blood using an episomal reprogramming method. Master cell bank or working cell bank are available.
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Applied StemCell’s cGMP Grade iPSC Line |
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| Source cell type: CD34+ Cord Blood, male | Reprogramming method: Episomal | Master cell bank or working cell bank available | Product QC:
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iPSC Expansion and Banking
ASC provides iPSC expansion and banking of its cGMP iPSCs. We also provide custom iPSC generation services using an epsiomal reprogramming method.
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cGMP iPSC Expansion and Banking |
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| Number of Vials | Cell Count | Other Deliverables | |
| Master Cell Bank | 50-200 vials | 1×10^6 cells/vial | QC and CoA |
| Working Cell Bank | 50-200 vials | 1×10^6 cells/vial | QC and CoA |
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Customized iPSC Generation (Episomal Method) |
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| Number of Clones | Number of Vials | Cell Count | Other Deliverables | |
| Seed Bank | 3 Clones | 10 vials | 1×10^6 cells/vial | QC Characterization
and CoA |
iPSC Characterization: Direct Differentiation to Three Germ Layers
Figure 1: Direct differentiation of ASE-9280 GMP Grade iPSCs to three germ layers. Staining for lineage-specific biomarkers of three germ layers after direct differentiation of the GMP hiPSC line, ASE-9280. The ASE-9280 GMP hiPSC line was differentiated to specific lineages of the germ layers using well-established and optimized protocols. Immunostaining for biomarkers of each lineage was performed. Cells were also co-stained with nuclear marker, DAPI (blue). Biomarkers: Ectoderm (EC) marker: Pax6 (green) and Tuji (red); Mesoderm (ME) marker: Brachyury (red) and GATA-4 (green); Endoderm (EN) marker: Fox2A (red) and Sox-17 (green).
Visit our cGMP page for more details about our cGMP services ->