TARGATT™ CHO Cells

Rapid, Stable CHO Cell Generation for Bioproduction

With our proprietary TARGATT™ technology for site-specific integration of large DNA fragments, we’ve developed a series of TARGATT™ “master” cell lines, including engineered CHO. These cell lines are part of our user-friendly TARGATT™ Knock-In Kits, which you can use to knock in your GOI in your own lab. Using our TARGATT™ CHO cell lines and kits, stable CHO cells containing your gene of interest can be generated as fast as in two weeks. The TARGATT™ CHO can also be used for library construction and screening for new antibodies.

Why choose TARGATT™ CHO Cells?

Chinese Hamster Ovary (CHO) cells have emerged as a popular and versatile choice for biopharmaceutical production and research applications, offering several distinct advantages. Their adaptability to genetic engineering is exemplified by proprietary technologies like TARGATT™, which enables site-specific integration of large DNA fragments. This capability allows for the creation of “master” cell lines that can be easily manipulated to express genes of interest (GOI) with high efficiency. CHO cells are particularly valued for bioproduction of antibodies for example. Together with our TARGATT™ site-specific gene insertion technology, stable CHO cells containing a gene of interest can be generated within two weeks, offering a cost-effective and fast approach for bioproduction. 

TARGATT™ CHO Master Cell Lines:
  • Site-specific knock in into a high expression, safe harbor locus (H11)
  • High efficiency integration >90% with drug selection
  • Stable gene insertion intead of transient gene expresion
  • BSL1 compatible
TARGATT™ CHO Library Kits
  • Large cell library construction in CHO cells
  • Development of a mammalian display system with a higher efficiency
  • Provide a mammalian display system that can reach E. coli and yeast library sizes

Get Started with Applied StemCell

Contact Information

Reach out to us directly through our contact page for any inquiries. Our team is ready to assist you with your TARGATT™ CHO cell needs.

Request a Quote

Requesting a quote is easy. Provide us with the details of your project, and we will deliver a comprehensive and competitive quote tailored to your specific requirements.

Technical Details

Figure 1: TARGATT™ Knock-In Strategy.  TARGATT™ technology enables fast and site-specific, stable integration of large DNA fragments (up to 22 kb) into an intergenic, transcriptionally active safe harbor locus. We can engineer an “attP” integrase recognition landing pad at a safe harbor locus. Single-copy gene insertion occurs when it is used in conjunction with an “attB” containing donor plasmid and integrase expression.

Support Materials

eBook:

TARGATT™ Technology For Antibody Discovery and Screening
(March 2021)
*Featured in Informa Connect’s eBook: Antibody
Discovery, Selection & Screening