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TARGATT™ HEK293 High-Efficiency Sub-Line (HESL) Knock-in Kit H11 for Library Screening (FACS)

TARGATT™ HEK293 High-Efficiency Sub-Line (HESL) Knock-in Kit H11 for Library Screening (FACS)

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TARGATT™ HEK293 High-Efficiency Sub-Line (HESL) Knock-in Kit (H11)

for Library Screening (FACS)

Insert confidently with our TARGATT™ Knock-in Kit:

  • High efficiency integration
  • Site-specific, stable knock-in cell line generation
  • Single copy gene integration into safe harbor locus
  • Gene expression from an active, intergenic locus
  • Low off-target integration

Overview

The TARGATT™ HEK293 High-Efficiency Sub-Line (HESL) Knock-in Kit (H11) for Library Screening (FACS) is designed for fast, efficient, and site-specific integration of DNA fragments of up to 20 kb into HEK293 cells. The master cell line provided in this kit contains the TARGATT™ landing pad with an attP site engineered into the H11 safe harbor locus. The kit also contains a cloning plasmid containing a corresponding “attB” sequence into which any gene of interest can be cloned. The expression of the integrase (accomplished via the provided plasmid) mediates stable integration of the transgene into the master cell line (Figure 1). The TARGATT™ integrase enables highly efficient (>60% without enrichment, >90% with enrichment), and site-specific DNA integration without disruption of internal genes.

The TARGATT™ HEK293 High-Efficiency Sub-Line (HESL) Knock-in Kit (H11) for Library Screening (FACS) is ideal for single-transgene-per-cell knock-in and uniform, stable expression of your protein.

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Our knock-in strategy

Figure 1. Schematic representation of TARGATT™ site-specific transgene integration mediated by integrase. The TARGATT™ HEK Master Cell Line was engineered with the attP landing pad at the H11 safe harbor locus. The TARGATT™ plasmid containing the integrase recognition site, attB is used to clone the transgene. The integrase catalyzes an irreversible reaction between the attP site in the genome and attB site in the donor vector, resulting in integration of the gene of interest at the selected H11 locus.

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