Reveal key players, identify and screen new therapeutic targets, and illuminate the molecular mechanisms underlying this complex disease
When you’re exploring the genetic drivers of cancer or screening for new therapeutic targets, having the right cell model can make all the difference. The experienced team at Applied StemCell can engineer that cell-based disease model for you, whether you need to activate or repress genes, introduce precise edits, or run large-scale CRISPR screens.
Our cancer cell line engineering services support your research with fully customized CRISPRa, CRISPRi, fusion protein-based editing, and library screening solutions. You’ll get expertly engineered cell lines built to match your project goals—so you can focus on making breakthroughs in oncology.
Here are just a few of the ways we can engineer a cancer cell line for your needs. If you don’t see what you’re looking for, simply contact us, we’ll get it done!
Learn about the Cas9 variants we use here.
Custom design
At Applied StemCell, wedesign specific gRNA constructs that target your genes of interest, optimizing for maximum repression efficiency.
Vector construction and cell line engineering
Our experts will use TARGATT™ technology to knock in dCas9-KRAB and sgRNA into the H11 safe harbor site. You’ll get consistent expression of dCas9-KRAB and your sgRNA.
Learn about TARGATT™ Technology here.
Alternatively, we can construct lentiviral vectors expressing dCas9-KRAB and sgRNAs for random integration into the genome.
Validation
Each cell line is subjected to extensive validation processes, including sequencing, gene expression analysis, and functional assays to guarantee CRISPRi activity and reliability.
Clonal selection
We isolate and expand single-cell clones, providing you with a homogenous population of high-quality CRISPRi-ready cells.
Custom design
At Applied StemCell, we can design specific gRNA constructs that precisely target your genes of interest, ensuring maximum activation efficiency tailored to your specific research goals.
Vector construction & cell line engineering
Our experts will use TARGATT™ technology to knock in dCas9-VPR and sgRNA into the H11 safe harbor site. You’ll get consistent expression of dCas9-VPR and your sgRNA.
Learn about TARGATT™ Technology here.
Alternatively, we can develop customized lentiviral vectors that express dCas9-VPR and sgRNAs for random insertion into the genome.
Validation
Each cell line is subjected to extensive validation processes including sequencing, gene expression analysis and functional assays to guarantee CRISPRa activity and reliability.
Clonal selection
We isolate and expand single-cell clones, providing you with a homogenous population of high-quality, CRISPRa-ready cells.
We offer two main types of libraries used for high-throughput screening: pooled libraries and array libraries.
Pooled libraries are cost-effective and provide comprehensive genome-wide coverage, making them suitable for in vivo studies. They involve the computer design of sgRNA libraries, which are then screened and enriched for positive clones.
Array libraries, on the other hand, are suitable for processing single or multiple sgRNA arrays in microarrays or multi-well plates for targeted applications.
Design and synthesis of sgRNA libraries
Applied StemCell provides genome-wide libraries containing over 100,000 sgRNAs to identify genes of interest in cancer and immortalized cell lines.
Vector construction & cell line engineering
Our experts will use TARGATT™ technology to knock your sgRNA into the H11 safe harbor site of a stable Cas9-expressing cell line.
Learn about TARGATT™ Technology here.
Screening strategy
Depending on the study’s purpose, either a positive or negative screening strategy is selected.
High-throughput sequencing and bioinformatics analysis
Bioinformatics methods are used to identify candidate genes and exclude false results.
Validation of candidate genes
Functional genes are validated through off-target analysis, gene knockout validation, and genetic complementation experiments.
By working with Applied StemCell, you experience deep technical expertise, flexibility, efficiency, and quality assurance, and gain a trusted partner committed to your success
Each of cell line we generate undergoes comprehensive quality control, including mycoplasma testing and authentication, ensuring the highest standards of research reliability.
With industry experience dating back to 2008 and over 500 distinct cell lines successfully edited, we have the expertise and experience to deliver what you need.
Our cutting-edge protocols are optimized for efficiency, minimizing your time and resources while maximizing research outcomes
All projects completed at our facilities in California (cell-based projects) and Oklahoma (animal projects)
Study disease in relevant cell types with full confidence that the phenotype you observe is not due to an off-target mutation, whether you are knocking in a functional gene, knocking out a gene, or exploring mutations in non-coding regions.
Explore gene function and map out gene interactions, genetic pathways, and complex gene networks with clarity and confidence.
Find out how our iPSC gene editing services can enable your projects by submitting the form below. We will get back to you within one business day.
