Gene Editing Platforms

Leverage the latest gene editing technologies and our exclusive innovations for your mammalian cell line engineering project

At Applied StemCell, our expert team offers services using a full suite of advanced and proprietary gene editing platforms to engineer the cell line you need.

Whether your project requires a precise point mutation, a complex knock-in, or stable expression of a large transgene, our team will select and apply the most effective gene editing technology for your goals. With access to exclusive tools like TARGATT™ and S-SELeCT™, as well as trusted systems like CRISPR/Cas9 and MAD7, we can tackle even the most challenging edits in mammalian cell lines.

Learn about the gene editing platforms we use—each one backed by extensive experience and a track record of success in custom cell line development.

1,000+

Gene editing projects completed 

>98%

Projects completed to customer specifications 

8/19

Patents granted/pending 

See what we can do for you with our gene editing platforms

Cell types* 

  • iPSCs (healthy or diseased) 
  • HEK293 
  • CHO 
  • Custom 
  • Research grade 
  • cGMP grade  
  • Matched research and cGMP grade 

Capabilities

  • Knock-out 
  • Knock-in (up to 20 kb) 
  • Point mutation or single-base editing 
  • Complex, multi-step cell line engineering 
  • Multi-gene knock-ins
  • Inducible gene expression
  • Reliable, consistent expression from the H11 safe harbor site
  • Footprint-free edits

Applications 

  • Disease modeling 
  • Tissue engineering 
  • Regenerative & cell-based medicine R&D 
  • Therapeutic R&D 
  • Basic research 
  • Library construction 
  • Promoter screening libraries
  • CRISPR screening libraries
  • Cell product cGMP manufacturing 

*We also offer CRISPR editing to generate rodent animal models — contact us to learn more.

Frequently asked questions

Didn’t find all the info you were looking for? 

Still have questions? 

No problem.

We work closely with you to understand your project’s objective(s) and ensure we accomplish your goals.

We can implement your designs and will also use our years of expertise to design an effective and efficient genome editing strategy, whether you require a simple edit or a complex construction.

While you can use CRISPR Cas9 technology for product development, the licensing requirements can be complex. For this reason, we recommend using Mad7 for any projects that will be used to develop a commercial product.

Yes, we are highly experienced in handling iPSCs and ensure that they retain pluripotency throughout the gene editing process.

The timeline for a gene editing project can vary greatly and depends on the complexity of the gene editing and whether you need to send in cells or can start with one of our hiPSC lines or TARGATT master cell lines. Tells us about your project and we can give you an estimate of the timeline—simply complete this form.

Yes! You can send in your cell lines or move faster by choosing one of our hiPSC lines or TARGATT master cell lines.

We were one of the first, if not the first, CROs to obtain a CRISPR Cas9 license for use in cells and rodents, back in 2014, highlighting our long experience and deep expertise performing gene edits in mammalian cells and rodents.

Get more information

Interested in starting a service or learning more about our process? Fill out the contact form below and a team member will be in touch within one business day.