ASE-9211

Human Induced Pluripotent Stem Cells (from Normal, Male, Human Fibroblasts)
Male hiPSC line, p15 MTA required for industry

Applied StemCell, Inc. provides Control Human Induced Pluripotent Stem (iPS) cells at low passages (p15). These pluripotent cells were generated from normal human skin fibroblasts using episomal reprogramming methods. This method allows the transient expression of human transcription factors (OCT4, SOX-2, KLF4, and c-MYC) that initiate the reprogramming process. The resulting human iPS cells (hiPSCs) were selected using morphological criteria without the use of either fluorescent markers or drug selection. These iPS cells have been tested for the expression of the pluripotency markers, including OCT4, SOX2, SSEA4, TRA-1-60, TRA-1-81, and alkaline phosphatase (AP) activity (Figure 1) and normal male karyotype (Figure 2). The ASE9211 control human iPSC line can be used for CRISPR/Cas9 genome editing and differentiation to somatic lineages in vitro. Detailed protocols for thawing, culturing under feeder-free conditions, and cryopreservation of these iPS cells are provided.