CRISPR (Cas9) Knock-in iPSC Service

Knock-in iPS Cell Generation Service Details

Service Advantages:

• Optimized CRISPR protocols for high success rate
• Footprint-free, feeder-free transfection and iPSC culture protocols
• Isogenic control lines for reliable experiments
• Knock-in a variety of genes for different applications
  • Locus-specific small/ large fragment gene insertions
  • Safe harbor locus knock-in
  • Reporter gene knock-in/ gene tagging
  • Gene overexpression (Ex. Antibody/ antigen expression)
  • Promoter modifications
  • Gene replacement; gene therapy
  • Gene fusion/ translocation

Standard Packaging:

• Ship 2 million frozen cells in cryovials (two (2) vials with 1 x 10^6 cells/vial)
  • iPSC Control Lines & iPSC Generation Services are available
• With pathogen and SARS-CoV-2 test results

Standard Deliverables:

• Two (2) clones that are homozygous for the targeted insertion, with two (2) vials of each clone at 1 x 10^6 cells/vial.
• Project Milestones Reports, as well as a Final Report containing details of targeting design, experimental, and genotyping results.

Standard Workflow and Timeline:

Workflow includes:

• Cell line evaluation
• gRNA design, CRISPR vector, and donor DNA construction, and reagent validation
• Transfection of targeting vectors and optimization
• Screening for single cell clones and clone confirmation
• Cell expansion and cryopreservation

Timeline: 3-4 months

Required Starting Material & Information

• Send us your healthy patient iPSC or ESC lines: Or
• Choose from our well-characterized control “master” iPSC lines; OR
• Send in your human or non-human samples and we will generate your iPSCs using our optimized reprogramming protocols
• Pathogen and SARS-CoV-2 test results

Applications:

• Physiologically relevant disease models for hard-to-model diseases (Ex. ALS, muscular dystrophy, Parkinson’s disease, Alzheimer’s)
• Differentiate to study mutations in different tissue lineages with an isogenic panel of cell line models
• Ideal for target drug discovery, drug and toxicity screening

Also available, downstream iPSC differentiation and characterization services.