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Reference Publications

SERVICE

CRISPR Cell Line Knock-Out, Knock-in, Point Mutation

  • Ioanna Boumpoureka; Vyron Gorgogietas; Elizabet Petkovski (2026). Generation of two isogenic control iPSC lines (LCSBi001-A-2 and LCSBi001-A-3) from a Parkinson’s disease patient line (LCSBi001-A) carrying the pathogenic VPS35 p.D620N mutation. Stem Cell Research, 92, 103944. 10.1016/j.scr.2026.103944.
  • Hamrick, Samantha K.; Kim, C.S. John; Tester, David J.; Gencarelli, Manuela; Tobert, Kathryn E.; Gluscevic, Martina; Ackerman, Michael J. (2024). Single Construct Suppression and Replacement Gene Therapy for the Treatment of All CALM1-, CALM2-, and CALM3-Mediated Arrhythmia Disorders. Circulation: Arrhythmia and Electrophysiology, 17, 8. 10.1161/CIRCEP.123.012036.
  • Boon, Nanda; Lu, Xuefei; Andriessen, Charlotte A.; Orlovà, Michaela; Quinn, Peter M. J.; Boon, Camiel J. F.; Wijnholds, Jan (2023). Characterization and AAV-mediated CRB gene augmentation in human-derived CRB1KO and CRB1KOCRB2+/− retinal organoids. Molecular Therapy Methods & Clinical Development, 31, 101128. 10.1016/j.omtm.2023.101128.
  • Rizo, Tania; Gebhardt, Lisa; Riedlberger, Julia; Eberhardt, Esther; Fester, Lars; Alansary, Dalia; Winkler, Jürgen; Turan, Soeren; Arnold, Philipp; Niemeyer, Barbara A; Fischer, Michael J M; Winner, Beate (2022). Store-operated calcium entry is reduced in spastin-linked hereditary spastic paraplegia. Brain, 145(9), 3131–3146. 10.1093/brain/awac122.
  • Hamrick, Samantha K.; John Kim, C. S.; Tester, David J.; Giudicessi, John R.; Ackerman, Michael J. (2022). Patient-specific, re-engineered cardiomyocyte model confirms the circumstance-dependent arrhythmia risk associated with the African-specific common SCN5A polymorphism p.S1103Y: Implications for the increased sudden deaths observed in black individuals during the COVID-19 pandemic. Heart Rhythm, 19(5), 822-827. 10.1016/j.hrthm.2021.12.029.
  • Peluso, M. O., Adam, A., Armet, C. M., Zhang, L., O’Connor, R. W., Lee, B. H., … & Palombella, V. J. (2020). The Fully human anti-CD47 antibody SRF231 exerts dual-mechanism antitumor activity via engagement of the activating receptor CD32a. Journal for ImmunoTherapy of Cancer, 8(1), e000413.
  • Tanic, J., Wang, Y., Lee, W., Coelho, N. M., Glogauer, M., & McCulloch, C. A. (2019). Adseverin modulates morphology and invasive function of MCF7 cells. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1865(10), 2716-2725.
  • Panda, D., Gjinaj, E., Bachu, M., Squire, E., Novatt, H., Ozato, K., & Rabin, R. L. (2019). IRF1 maintains optimal constitutive expression of antiviral genes and regulates the early antiviral response. Frontiers in immunology, 10, 1019.
  • Pisapia, P., Malapelle, U., Roma, G., Saddar, S., Zheng, Q., Pepe, F., … & Nikiforov, Y. E. (2019). Consistency and reproducibility of next‐generation sequencing in cytopathology: A second worldwide ring trial study on improved cytological molecular reference specimens. Cancer cytopathology, 127(5), 285-296.
  • Ilic, D. (2019). Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in October 2018. Regenerative medicine, 14(2), 85-92.
  • Simkin, D., Searl, T. J., Piyevsky, B. N., Forrest, M., Williams, L. A., Joshi, V., … & Penzes, P. (2019). Impaired M-current in KCNQ2 Encephalopathy Evokes Dyshomeostatic Modulation of Excitability. bioRxiv, 538371. https://doi.org/10.1101/538371
  • Jang, Y., Choi, J., Park, N., Kang, J., Kim, M., Kim, Y., & Ju, J. H. (2019). Development of immunocompatible pluripotent stem cells via CRISPR-based human leukocyte antigen engineering. Experimental & Molecular Medicine, 51(1), 3.
  • Colomar-Carando, N., Meseguer, A., Jutz, S., Herrera-Fernández, V., Olvera, A., Kiefer, K., … & Vicente, R. (2018). Zip6 Transporter Is an Essential Component of the Lymphocyte Activation Machinery. The Journal of Immunology, ji1800689.
  • Tanic, J. (2018). A Role for Adseverin in the Invasion and Migration of MCF7 Breast Adenocarcinoma Cells (Doctoral dissertation).
  • Lizarraga, S. B., Maguire, A. M., Ma, L., van Dyck, L. I., Wu, Q., Nagda, D., … & Cowen, M. H. (2018). Human neurons from Christianson syndrome iPSCs reveal allele-specific responses to rescue strategies. bioRxiv, 444232.
  • Tanaka, H., Kondo, K., Chen, X., Homma, H., Tagawa, K., Kerever, A., … & Fujita, K. (2018). The intellectual disability gene PQBP1 rescues Alzheimer’s disease pathology. Molecular Psychiatry, 1.
  • Yin, Y., Garcia, M. R., Novak, A. J., Saunders, A. M., Ank, R. S., Nam, A. S., & Fisher, L. W. (2018). Surf4 (Erv29p) binds amino-terminal tripeptide motifs of soluble cargo proteins with different affinities, enabling prioritization of their exit from the endoplasmic reticulum. PLoS biology, 16(8), e2005140.
  • Selvan, N., George, S., Serajee, F. J., Shaw, M., Hobson, L., Kalscheuer, V. M., … & Schwartz, C. E. (2018). O-GlcNAc transferase missense mutations linked to X-linked intellectual disability deregulate genes involved in cell fate determination and signaling. Journal of Biological Chemistry, jbc-RA118.
  • Smalley, E. (2018). FDA warns public of dangers of DIY gene therapy. https://doi.org/10.1038/nbt0218-119
  • Chai, S., Wan, X., Ramirez-Navarro, A., Tesar, P. J., Kaufman, E. S., Ficker, E., … & Deschênes, I. (2018). Physiological genomics identifies genetic modifiers of long QT syndrome type 2 severity. The Journal of clinical investigation, 128(3).
  • Boi, S., Ferrell, M. E., Zhao, M., Hasenkrug, K. J., & Evans, L. H. (2018). Mouse APOBEC3 expression in NIH 3T3 cells mediates hypermutation of AKV murine leukemia virus. Virology, 518, 377-384. https://doi.org/10.1016/j.virol.2018.03.014.
  • Molinski, S. V., et al. (2017). Orkambi® and amplifier co‐therapy improves function from a rare CFTR mutation in gene‐edited cells and patient tissue. EMBO Molecular Medicine, e201607137.
  • Petrovic, P. B. (2017). Myosin Phosphatase Rho-interacting Protein Regulates DDR1-mediated Collagen Tractional Remodeling (Doctoral dissertation, University of Toronto (Canada)).
  • Peng, L., Zhang, H., Hao, Y., Xu, F., Yang, J., Zhang, R., … & Chen, C. (2016). Reprogramming macrophage orientation by microRNA 146b targeting transcription factor IRF5. EBioMedicine, 14, 83-96.
  • Hu, J. K., Crampton, J. C., Locci, M., & Crotty, S. (2016). CRISPR-mediated Slamf1Δ/Δ Slamf5Δ/Δ Slamf6Δ/Δ triple gene disruption reveals NKT cell defects but not T follicular helper cell defects. PloS one, 11(5), e0156074.
  • Smalley, E. (2016). CRISPR mouse model boom, rat model renaissance. Nature Biotechnology. 34, 893–894.
  • Baker, M. (2014). Gene editing at CRISPR speed. Nature biotechnology, 32(4), 309-313.

TARGATT™ Site-Specific Knock-in Cell Lines

Book Chapters

Master Cell Line

  • Chi, X., Zheng, Q., Jiang, R., Chen-Tsai, R. Y., & Kong, L. J. (2019). A system for site-specific integration of transgenes in mammalian cells. PLOS ONE, 14(7), e0219842.

Description of the technology

  • Zhu, F., Gamboa, M., Farruggio, A. P., Hippenmeyer, S., Tasic, B., Schüle, B., … Calos, M. P. (2014). DICE, an efficient system for iterative genomic editing in human pluripotent stem cells. Nucleic Acids Research, 42(5), e34. http://doi.org/10.1093/nar/gkt1290.
  • Tasic, B., Hippenmeyer, S., Wang, C., Gamboa, M., Zong, H., Chen-Tsai, Y., & Luo, L. (2011). Site-specific integrase-mediated transgenesis in mice via pronuclear injection. Proceedings of the National Academy of Sciences of the United States of America, 108(19), 7902–7907. http://doi.org/10.1073/pnas.1019507108.

Commentary, comparison with other transgenic methods

  • Rossant, J., Nutter, L. M., & Gertsenstein, M. (2011). Engineering the embryo. Proceedings of the National Academy of Sciences, 108(19), 7659-7660.

Advantage of Hipp11 (H11) locus

iPSC Generation

  • Benedetti, Maria Cristina; D’andrea, Tiziano; Colantoni, Alessio; Silachev, Denis; Turris, Valeria de; Boussadia, Zaira; Babenko, Valentina A.; Volovikov, Egor A.; Belikova, Lilia; Bogomazova, Alexandra N.; Pepponi, Rita; Whye, Dosh; Buttermore, Elizabeth D.; Tartaglia, Gian Gaetano; Lagarkova, Maria A.; Katanaev, Vladimir L.; Musayev, Ilya; Martinelli, Simone; Fucile, Sergio; Rosa, Alessandro (2024). Cortical neurons obtained from patient-derived iPSCs with GNAO1 p.G203R variant show altered differentiation and functional properties. Heliyon, 10(5), e26656. 10.1016/j.heliyon.2024.e26656.
  • Kim, Maengjo; Das, Saumya; Tester, David J.; Pradhananga, Sabindra; Hamrick, Samantha K.; Gao, Xiaozhi; Srinivasan, Dinesh; Sager, Philip T.; Ackerman, Michael J. (2023). SGK1 inhibition attenuated the action potential duration in patient- and genotype-specific re-engineered heart cells with congenital long QT syndrome. Heart Rhythm O2, 4(4), 268-274. 10.1016/j.hroo.2023.02.003.
  • Asher, Melissa J.; McMullan, Hannah M.; Dong, Ao; Li, Yulong; Thayer, Stanley A. (2023). A Complete Endocannabinoid Signaling System Modulates Synaptic Transmission between Human Induced Pluripotent Stem Cell–Derived Neurons. Molecular Pharmacology, 103(2), 100-112. 10.1124/molpharm.122.000555.
  • Mace, E. M., Paust, S., Conte, M. I., Baxley, R. M., Schmit, M., Mukherjee, M., … & Akdemir, Z. C. (2019). Human NK cell deficiency as a result of biallelic mutations in MCM10. bioRxiv, 825554.
  • Baskfield, A., Li, R., Beers, J., Zou, J., Liu, C., & Zheng, W. (2019). Generation of an induced pluripotent stem cell line (TRNDi004-I) from a Niemann-Pick disease type B patient carrying a heterozygous mutation of p. L43_A44delLA in the SMPD1 gene. Stem cell research, 37, 101436.
  • Hong, J., Xu, M., Li, R., Cheng, Y. S., Kouznetsova, J., Beers, J., … & Zheng, W. (2019). Generation of an induced pluripotent stem cell line (TRNDi008-A) from a Hunter syndrome patient carrying a hemizygous 208insC mutation in the IDS gene. Stem cell research, 37, 101451.
  • Cheng, Y. S., Li, R., Baskfield, A., Beers, J., Zou, J., Liu, C., & Zheng, W. (2019). A human induced pluripotent stem cell line (TRNDi007-B) from an infantile onset Pompe patient carrying p. R854X mutation in the GAA gene. Stem cell research, 37, 101435.
  • Yang, S., Cheng, Y. S., Li, R., Pradhan, M., Hong, J., Beers, J., … & Zheng, W. (2019). An induced pluripotent stem cell line (TRNDi010-C) from a patient carrying a homozygous p. R401X mutation in the NGLY1 gene. Stem cell research, 39, 101496.
  • Baskfield, A., Li, R., Beers, J., Zou, J., Liu, C., & Zheng, W. (2019). An induced pluripotent stem cell line (TRNDi009-C) from a Niemann-Pick disease type A patient carrying a heterozygous p. L302P (c. 905 T> C) mutation in the SMPD1 gene. Stem cell research, 38, 101461.
  • Huang, W., Xu, M., Li, R., Baskfield, A., Kouznetsova, J., Beers, J., … & Zheng, W. (2019). An induced pluripotent stem cell line (TRNDi006-A) from a MPS IIIB patient carrying homozygous mutation of p. Glu153Lys in the NAGLU gene. Stem Cell Research, 101427.
  • Sundararaj, K. P., Rodgers, J., Angel, P., Wolf, B., & Nowling, T. K. (2020). Neuraminidase activity mediates IL-6 production through TLR4 and p38/ERK MAPK signaling in MRL/lpr mesangial cells. bioRxiv.
  • Li, R., Baskfield, A., Lin, Y., Beers, J., Zou, J., Liu, C., … & Zheng, W. (2019). Generation of an induced pluripotent stem cell line (TRNDi003-A) from a Noonan syndrome with multiple lentigines (NSML) patient carrying a p. Q510P mutation in the PTPN11 gene. Stem cell research, 34, 101374.
  • Allende, Maria L.; Cook, Emily K.; Larman, Bridget C.; Nugent, Adrienne; Brady, Jacqueline M.; Golebiowski, Diane; Sena-Esteves, Miguel; Tifft, Cynthia J.; Proia, Richard L. (2018). Cerebral organoids derived from Sandhoff disease-induced pluripotent stem cells exhibit impaired neurodifferentiation[S]. Journal of Lipid Research, 59(3), 550-563. 10.1194/jlr.M081323.
  • Li, R., Pradhan, M., Xu, M., Baskfield, A., Farkhondeh, A., Cheng, Y. S., … & Rodems, S. (2018). Generation of an induced pluripotent stem cell line (TRNDi002-B) from a patient carrying compound heterozygous p. Q208X and p. G310G mutations in the NGLY1 gene. Stem Cell Research, 101362.
  • Poli, M. C., Ebstein, F., Nicholas, S. K., de Guzman, M. M., Forbes, L. R., Chinn, I. K., … & Coban-Akdemir, Z. H. (2018). Heterozygous Truncating Variants in POMP Escape Nonsense-Mediated Decay and Cause a Unique Immune Dysregulatory Syndrome. The American Journal of Human Genetics, 102, 1-17. https://doi.org/10.1016/j.ajhg.2018.04.010
  • Vozdek, R., Long, Y., & Ma, D. K. (2018). The receptor tyrosine kinase HIR-1 coordinates HIF-independent responses to hypoxia and extracellular matrix injury. Sci. Signal., 11(550), eaat0138

Vector Design/ Cloning Service (*cited/published articles)

  • Hu, Q., Li, C., Wang, S., Li, Y., Wen, B., Zhang, Y., … & Nguyen, T. K. (2019). LncRNAs-directed PTEN enzymatic switch governs epithelial–mesenchymal transition. Cell research, 1.
  • Besschetnova, T. Y., Ichimura, T., Katebi, N., Croix, B. S., Bonventre, J. V., & Olsen, B. R. (2015). Regulatory mechanisms of anthrax toxin receptor 1-dependent vascular and connective tissue homeostasis. Matrix Biology, 42, 56-73.

Lentivirus Packaging

Vector Design/ Cloning Service (*cited/published articles)

  • Hu, Q., Li, C., Wang, S., Li, Y., Wen, B., Zhang, Y., … & Nguyen, T. K. (2019). LncRNAs-directed PTEN enzymatic switch governs epithelial–mesenchymal transition. Cell research, 1.
  • Besschetnova, T. Y., Ichimura, T., Katebi, N., Croix, B. S., Bonventre, J. V., & Olsen, B. R. (2015). Regulatory mechanisms of anthrax toxin receptor 1-dependent vascular and connective tissue homeostasis. Matrix Biology, 42, 56-73.

Lentivirus Packaging

TARGATT iPSC Applications

  • S. Wu; D. Nonis; Y. Cho; Q. Zhu; A. Farruggio; R. Tsai (2025). Generating a Hypoimmune iPSC Line to Enable Allogeneic Therapies. Cytotherapy, 27(5), Supplement, S243. 10.1016/j.jcyt.2025.03.503.

TARGATT Site-Specific Knock-In Cell Lines

  • Chi, X.; Zheng, Q.; Jiang, R.; Chen-Tsai, R. Y.; Kong, L. J. (2019). A system for site-specific integration of transgenes in mammalian cells. PLOS ONE, 14(7), e0219842. 10.1371/journal.pone.0219842.

TARGATT Supporting Technology

  • Zhu, F.; Gamboa, M.; Farruggio, A. P.; Hippenmeyer, S.; Tasic, B.; et al. (2014). DICE, an efficient system for iterative genomic editing in human pluripotent stem cells. Nucleic Acids Research, 42(5), e34. 10.1093/nar/gkt1290. 

PRODUCTS

CRISPR Cell Line Knock-Out, Knock-in, Point Mutation

In vivo validated Cas9 mRNAs

ASE-9109

  • Melo de Farias, Ana Raquel; Pelletier, Alexandre; Iohan, Lukas Cruz Carvalho; Saha, Orthis; Bonnefond, Amélie; Amouyel, Philippe; Delahaye, Fabien; Lambert, Jean-Charles; Costa, Marcos R. (2023). Amyloid-Beta Peptides Trigger Premature Functional and Gene Expression Alterations in Human-Induced Neurons. Biomedicines, 11(9), 2564. 10.3390/biomedicines11092564.
  • Dotzler, Steven M.; Kim, C.S. John; Gendron, William A.C.; Zhou, Wei; Ye, Dan; Bos, J. Martijn; Tester, David J.; Barry, Michael A.; Ackerman, Michael J. (2021). Suppression-Replacement KCNQ1 Gene Therapy for Type 1 Long QT Syndrome. Circulation, 143, 14. 10.1161/CIRCULATIONAHA.120.051836.

Human Primary Cell (Normal)

ASE-5168

  • Brodaczewska, K. K., Szczylik, C., Fiedorowicz, M., Porta, C., & Czarnecka, A. M. (2016). Choosing the right cell line for renal cell cancer research. Molecular cancer, 15(1), 83.

ASE-5063 – Human Kidney Epithelial Cells (Healthy)

  • Matak, D., Brodaczewska, K. K., Lipiec, M., Szymanski, Ł., Szczylik, C., & Czarnecka, A. M. (2017). Colony, hanging drop, and methylcellulose three dimensional hypoxic growth optimization of renal cell carcinoma cell lines. Cytotechnology, 1-14.
  • Matak, D., Brodaczewska, K. K., Szczylik, C., Koch, I., Myszczyszyn, A., Lipiec, M., … & Czarnecka, A. M. (2017). Functional significance of CD105-positive cells in papillary renal cell carcinoma. BMC cancer, 17(1), 21.
  • Khan, M. I., Czarnecka, A. M., Lewicki, S., Helbrecht, I., Brodaczewska, K., Koch, I., … & Szczylik, C. (2016). Comparative gene expression profiling of primary and metastatic renal cell carcinoma stem cell-like cancer cells. PloS one, 11(11), e0165718.

ASE-7001

  • Samura, M., Morikage, N., Suehiro, K., Tanaka, Y., Nakamura, T., Nishimoto, A., … Hamano, K. (2016). Combinatorial Treatment with Apelin-13 Enhances the Therapeutic Efficacy of a Preconditioned Cell-Based Therapy for Peripheral Ischemia. Scientific Reports, 6, 19379. http://doi.org/10.1038/srep19379.

ASE-9202

  • Corti, Serena; Kim, Kwi Hye; Chen, Ting; Botezatu, Adelina; Cora, Virginia; Ma, Ke; Pashkovskaia, Natalia; Vergara, Anamaria Bernal; Sperlich, Denise; Dave, Kaushambee; Tolone, Arianna; Reddinger, Ryan M.; Tully, Christopher B.; Higgins, Mikayla; Kleger, Alexander; Breunig, Markus; Lopatta, Paul; Wingerter, Svenja; Cipriano, Madalena; Bolz, Sylvia; Ueffing, Marius; Buss, Nicholas; Loskill, Peter; Liebau, Stefan; Achberger, Kevin (2025). Recreating pathophysiology of CLN2 disease and demonstrating reversion by TPP1 gene therapy in hiPSC-derived retinal organoids and retina-on-chip. Cell Reports Medicine, 6(8), 102244. 10.1016/j.xcrm.2025.102244.

ASE-9209

  • Wood, Aleksandar R.; Foliaki, Simote T.; Groveman, Bradley R.; Walters, Ryan O.; Williams, Katie; Yuan, Jue; Zou, Wen-Quan; Haigh, Cathryn L. (2022). Hereditary E200K mutation within the prion protein gene alters human iPSC derived cardiomyocyte function. Scientific Reports, 12, 15788. 10.1038/s41598-022-19631-5.

Human Skin Fibroblast Total RNA

  • Fujiwara, R., Takenaka, S., Hashimoto, M., Narawa, T., & Itoh, T. (2014). Expression of human solute carrier family transporters in skin: possible contributor to drug-induced skin disorders. Scientific Reports, 4, 5251. http://doi.org/10.1038/srep05251
  • Sumida, K., Kawana, M., Kouno, E., Itoh, T., Takano, S., Narawa, T., … Fujiwara, R. (2013). Importance of UDP-Glucuronosyltransferase 1A1 Expression in Skin and Its Induction by UVB in Neonatal Hyperbilirubinemia. Molecular Pharmacology, 84(5), 679–686. http://doi.org/10.1124/mol.113.088112.
  • Takenaka, S., Itoh, T., & Fujiwara, R. (2013). Expression pattern of human ATP‐binding cassette transporters in skin. Pharmacology research & perspectives, 1(1).

Total RNA-ASD-0088

Human Cardiomyocytes Total RNA-ASD-0061

  • Son, M. Y., et al. (2017). Transcriptome dynamics of human pluripotent stem cell-derived contracting cardiomyocytes using an embryoid body model with fetal bovine serum. Mol. BioSyst., doi:10.1039/C7MB00174F.
  • Jung, K. B., Son, Y. S., Lee, H., Jung, C. R., Kim, J., & Son, M. Y. (2017). Transcriptome dynamics of human pluripotent stem cell-derived contracting cardiomyocytes using an embryoid body model with fetal bovine serum. Molecular BioSystems.

HEK293T cells

Murine Endothelial cells

  • MAPK Mutation Panel FFPE Reference Standards

    •  Akinleye A, Furqan M, Mukhi N, Ravella P, Liu D (2013) MEK and the inhibitors: from bench to bedside. J. Hematol. Oncol. 6, 1–11
    • Santarpia L, Lippman SM, El-Naggar AK (2012) Targeting the MAPK–RAS–RAF signaling pathway in cancer therapy, Expert Opin. Ther. Targets 16, 103–119
    • Relling MV, Evans WE (2015) Pharmacogenomics in the clinic, Nature 526, 343–350
       

  • MEF Feeder Cells

    DR4 MEF Feeder Cells

    • Ruiz-Gutierrez, M., Bölükbaşı, Ö. V., Alexe, G., Kotini, A. G., Ballotti, K., Joyce, C. E., … & Papapetrou, E. P. (2019). Therapeutic discovery for marrow failure with MDS predisposition using pluripotent stem cells. JCI insight, 4(12).
    • Wagner, M., Yoshihara, M., Douagi, I., Damdimopoulos, A., Panula, S., Petropoulos, S., … & Hovatta, O. (2019). Single cell map of the human ovarian cortex. bioRxiv, 791343.
    • Takahashi, M., & Yamazaki, S. (2019). Generation of a human induced pluripotent stem cell line, IMSUTi002-A-1, harboring the leukemia-specific fusion gene ETV6-RUNX1. Stem cell research, 40, 101546.
    • Gruzdev, A., Scott, G. J., Hagler, T. B., & Ray, M. K. (2019). CRISPR/Cas9-Assisted Genome Editing in Murine Embryonic Stem Cells. In Mouse Models of Innate Immunity. Humana Press, New York, NY. 1690:1-21.
    • Snijders, K. E., Cooper, J. D., Vallier, L., & Bertero, A. (2019). Conditional Gene Knockout in Human Cells with Inducible CRISPR/Cas9. In: Luo Y. (eds) CRISPR Gene Editing. Methods in Molecular Biology, Humana Press, New York, NY. 1961:185-209.
    • Tan, C. E. H. (2018). Establishing a genetically engineered mouse ES cell line expressing an inducible Xist transgene along chromosome 19 (Doctoral dissertation).
    • Fogarty, N. M., McCarthy, A., Snijders, K. E., Powell, B. E., Kubikova, N., Blakeley, P., … & Maciulyte, V. (2017). Genome editing reveals a role for OCT4 in human embryogenesis. Nature, 550(7674), 67-73.
    • Molokanova, O., Schönig, K., Weng, S. Y., Wang, X., Bros, M., Diken, M., … & Eshkind, L. (2017). Inducible knockdown of procollagen I protects mice from liver fibrosis and leads to dysregulated matrix genes and attenuated inflammation. Matrix Biology. https://doi.org/10.1016/j.matbio.2017.11.002.
    • Marttila, S. (2017). Establishment and characterisation of new human induced pluripotent stem cell lines and cardiomyocyte differentiation: a comparative view. Master’s Thesis, University of Tampere, May 2017.
    • Honda, A., Kawano, Y., Izu, H., Choijookhuu, N., Honsho, K., Nakamura, T., … & Sankai, T. (2017). Discrimination of stem cell status after subjecting cynomolgus monkey pluripotent stem cells to naive conversion. Scientific reports, 7, 45285.
    • Friedel, T., Jung-Klawitter, S., Sebe, A., Schenk, F., Modlich, U., Ivics, Z., … & Schneider, I. C. (2016). CD30 Receptor-Targeted Lentiviral Vectors for Human Induced Pluripotent Stem Cell-Specific Gene Modification. Stem cells and development, 25(9), 729-739.
    • Ludwig, M., Kitzenberg, D., & Chick, W. S. (2015). Forward Genetic Approach to Uncover Stress Resistance Genes in Mice—A High-throughput Screen in ES Cells. Journal of visualized experiments: JoVE, (105).
    • Neri, T., Muggeo, S., Paulis, M., Caldana, M. E., Crisafulli, L., Strina, D., … & Scanziani, E. (2015). Targeted gene correction in osteopetrotic-induced pluripotent stem cells for the generation of functional Osteoclasts. Stem cell reports, 5(4), 558-568.
    • Kraus, P., Sivakamasundari, V., Xing, X., & Lufkin, T. (2014). Generating mouse lines for lineage tracing and knockout studies. In Mouse Genetics(pp. 37-62). Humana Press, New York, NY.
    • Zhu, F., Gamboa, M., Farruggio, A. P., Hippenmeyer, S., Tasic, B., Schüle, B., … & Calos, M. P. (2013). DICE, an efficient system for iterative genomic editing in human pluripotent stem cells. Nucleic acids research, 42(5), e34-e34.
    • Ivics, Z., Izsvák, Z., Chapman, K. M., & Hamra, F. K. (2011). Sleeping Beauty transposon mutagenesis of the rat genome in spermatogonial stem cells. Methods, 53(4), 356-365.
    • Ivics, Z., Izsvák, Z., Medrano, G., Chapman, K. M., & Hamra, F. K. (2011). Sleeping Beauty transposon mutagenesis in rat spermatogonial stem cells. Nature protocols, 6(10), 1521.
    • Pan, Y. (2011). Culturing of C57BL/6 Mouse Embryonic Stem (ES) Cell Line. Bio-protocol Bio101: e142. DOI: 10.21769/BioProtoc.142.
    • Chapman, K. M., Saidley-Alsaadi, D., Syvyk, A. E., Shirley, J. R., Thompson, L. M., & Hamra, F. K. (2011). Rat spermatogonial stem cell-mediated gene transfer. In Advanced Protocols for Animal Transgenesis (pp. 237-266). Springer, Berlin, Heidelberg.

    CF-1 MEF Feeder Cells

    • Thakurela, S., Sindhu, C., Yurkovsky, E., Riemenschneider, C., Smith, Z. D., Nachman, I., & Meissner, A. (2019). Differential regulation of OCT4 targets facilitates reacquisition of pluripotency. Nature communications, 10(1), 1-11.
    • Smela, M. P., Sybirna, A., Wong, F. C., & Surani, M. A. (2019). Testing the role of SOX15 in human primordial germ cell fate. Wellcome open research, 4.
    • Spada, F., Schiffers, S., Kirchner, A., Zhang, Y., Kosmatchev, O., Korytiakova, E., … & Carell, T. (2019). Oxidative and non-oxidative active turnover of genomic methylcytosine in distinct pluripotent states. BioRxiv, 846584.
    • Kiamehr, M., Klettner, A., Richert, E., Koskela, A., Koistinen, A., Skottman, H., … & Juuti-Uusitalo, K. (2019). Compromised Barrier Function in Human Induced Pluripotent Stem-Cell-Derived Retinal Pigment Epithelial Cells from Type 2 Diabetic Patients. International journal of molecular sciences, 20(15), 3773.
    • Barber, K., Studer, L., & Fattahi, F. (2019). Derivation of enteric neuron lineages from human pluripotent stem cells. Nature protocols, 14:1261–1279.
    • Berecz, T., Husvéth-Tóth, M., Mioulane, M., Merkely, B., Apáti, Á., & Földes, G. (2019). Generation and Analysis of Pluripotent Stem Cell-Derived Cardiomyocytes and Endothelial Cells for High Content Screening Purposes. In: Methods in Molecular Biology. Humana Press.
    • Madak-Erdogan, Z., Band, S., Zhao, Y. C., Smith, B. P., Kulkoyluoglu-Cotul, E., Zuo, Q., … & Kim, S. H. (2019). Free fatty acids rewire cancer metabolism in obesity-associated breast cancer via estrogen receptor and mTOR signaling. Cancer research, canres-2849.
    • Deuse, T., Hu, X., Gravina, A., Wang, D., Tediashvili, G., De, C., … & Davis, M. M. (2019). Hypoimmunogenic derivatives of induced pluripotent stem cells evade immune rejection in fully immunocompetent allogeneic recipients. Nature biotechnology, 1.
    • Kiamehr, M. (2019). Induced pluripotent stem cell-derived hepatocyte-like cells: The lipid status in differentiation, functionality, and de-differentiation of hepatic cells. Tampere University Dissertations.
    • Yeom, K. H., Mitchell, S., Linares, A. J., Zheng, S., Lin, C. H., Wang, X. J., … & Black, D. L. (2018). Polypyrimidine Tract Binding Protein blocks microRNA-124 biogenesis to enforce its neuronal specific expression. bioRxiv, 297515. https://doi.org/10.1101/297515
    • Chai, S., Wan, X., Ramirez-Navarro, A., Tesar, P. J., Kaufman, E. S., Ficker, E., … & Deschênes, I. (2018). Physiological genomics identifies genetic modifiers of long QT syndrome type 2 severity. The Journal of Clinical Investigation, 128(3). DOI: 10.1172/JCI94996
    • Oh, Y., Zhang, F., Wang, Y., Lee, E. M., Choi, I. Y., Lim, H., … & Wu, H. (2017). Zika virus directly infects peripheral neurons and induces cell death. Nature Neuroscience, 20(9), 1209-1212.
    • Kiamehr, M., Viiri, L. E., Vihervaara, T., Koistinen, K. M., Hilvo, M., Ekroos, K., … & Aalto-Setälä, K. (2017). Lipidomic profiling of patient-specific induced pluripotent stem cell-derived hepatocyte-like cells. Disease Models & Mechanisms, dmm-030841.
    • Wong, K. G., et al. (2017). CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells. http://www.cell.com/stem-cell-reports/pdfExtended/S2213-6711(17)30217-5.
    • Cvetkovic, C., et al. (2017). A 3D-printed platform for modular neuromuscular motor units. Microsystems & Nanoengineering, 3, 17015.
    • Kurapati, S., et al. (2017). Role of JNK pathway in varicella-zoster virus lytic infection and reactivation. Journal of Virology, JVI-00640.
    • Kotini, A. G., Chang, C. J., Chow, A., Yuan, H., Ho, T. C., Wang, T., … & Teruya-Feldstein, J. (2017). Stage-specific human induced pluripotent stem cells map the progression of myeloid transformation to transplantable leukemia. Cell Stem Cell, 20(3), 315-328.
    • Maghen, L., Shlush, E., Gat, I., Filice, M., Barretto, T. A., Jarvi, K., … & Librach, C. L. (2017). Human umbilical perivascular cells (HUCPVCs): a novel source of mesenchymal stromal-like (MSC) cells to support the regeneration of the testicular niche. Reproduction, 153(1), 85-95.
    • Nguyen, T. T. T., Park, W. S., Park, B. O., Kim, C. Y., Oh, Y., Kim, J. M., … & Hahn, K. M. (2016). PLEKHG3 enhances polarized cell migration by activating actin filaments at the cell front. Proceedings of the National Academy of Sciences, 113(36), 10091-10096.
    • Uzel, S. G., Platt, R. J., Subramanian, V., Pearl, T. M., Rowlands, C. J., Chan, V., … & Kamm, R. D. (2016). Microfluidic device for the formation of optically excitable, three-dimensional, compartmentalized motor units. Science advances, 2(8), e1501429.
    • Wang, J., Singh, M., Sun, C., Besser, D., Prigione, A., Ivics, Z., … & Izsvák, Z. (2016). Isolation and cultivation of naive-like human pluripotent stem cells based on HERVH expression. Nature protocols, 11(2), 327.
    • Mimura, S., Suga, M., Okada, K., Kinehara, M., Nikawa, H., & Furue, M. K. (2016). Bone morphogenetic protein 4 promotes craniofacial neural crest induction from human pluripotent stem cells. International Journal of Developmental Biology, 60(1-2-3), 21-28.
    • Kaini, R. R., Shen-Gunther, J., Cleland, J. M., Greene, W. A., & Wang, H. C. (2016). Recombinant xeno-free vitronectin supports self-renewal and pluripotency in protein-induced pluripotent stem cells. Tissue Engineering Part C: Methods, 22(2), 85-90.
    • Chambers, S. M., Mica, Y., Lee, G., Studer, L., & Tomishima, M. J. (2013). Dual-SMAD inhibition/WNT activation-based methods to induce neural crest and derivatives from human pluripotent stem cells. In Human Embryonic Stem Cell Protocols (pp. 329-343). Humana Press, New York, NY.
    • Nakshatri, H., Anjanappa, M., & Bhat-Nakshatri, P. (2015). Ethnicity-dependent and-independent heterogeneity in healthy normal breast hierarchy impacts tumor characterization. Scientific reports, 5, 13526.
    • Romanazzo, S., Forte, G., Morishima, K., & Taniguchi, A. (2015). IL-12 involvement in myogenic differentiation of C2C12 in vitro. Biomaterials science, 3(3), 469-479.
    • Ji, J., Zheng, X., Forgues, M., Yamashita, T., Wauthier, E. L., Reid, L. M., Wen, X., Song, Y., Wei, J. S., Khan, J., Thorgeirsson, S. S., … Wang, X. W. (2015). Identification of microRNAs specific for epithelial cell adhesion molecule-positive tumor cells in hepatocellular carcinoma. Hepatology (Baltimore, Md.), 62(3), 829-40.
    • Linares, A. J., Lin, C. H., Damianov, A., Adams, K. L., Novitch, B. G., & Black, D. L. (2015). The splicing regulator PTBP1 controls the activity of the transcription factor Pbx1 during neuronal differentiation. Elife, 4, e09268.
    • Elo, T. (2014). Evaluation of the pluripotency of human induced pluripotent stem cells (hiPSCs) reprogrammed with integrative and non-integrative protocols and their differentiation into cardiomyocytes (Master’s thesis).
    • Kraus, P., Sivakamasundari, V., Xing, X., & Lufkin, T. (2014). Generating mouse lines for lineage tracing and knockout studies. In Mouse Genetics(pp. 37-62). Humana Press, New York, NY.
    • Fattahi, F., et al. (2014) Neural Crest Cells from Dual SMAD Inhibition. Current protocols in stem cell biology, 1H-9.
    • Xu, Z., et al. (2013) PLoS One, 8(1), e53146.
    • Guo, X., Disatnik, M.-H., Monbureau, M., Shamloo, M., Mochly-Rosen, D., & Qi, X. (2013). Inhibition of mitochondrial fragmentation diminishes Huntington’s disease–associated neurodegeneration. The Journal of Clinical Investigation, 123(12), 5371–5388. http://doi.org/10.1172/JCI70911
    • Zhao, H., Sun, N., Young, S. R., Nolley, R., Santos, J., Wu, J. C., & Peehl, D. M. (2013). Induced pluripotency of human prostatic epithelial cells. PLoS One, 8(5), e64503.
    • Jerebtsova, M., Kumari, N., Xu, M., Melo, G. B. A. D., Niu, X., Jeang, K. T., & Nekhai, S. (2012). HIV-1 resistant CDK2-knockdown macrophage-like cells generated from 293T cell-derived human induced pluripotent stem cells. Biology, 1(2), 175-195.
    • Linta, L., Stockmann, M., Kleinhans, K. N., Böckers, A., Storch, A., Zaehres, H., … & Liebau, S. (2011). Rat embryonic fibroblasts improve reprogramming of human keratinocytes into induced pluripotent stem cells. Stem cells and development, 21(6), 965-976.
    • Meng, X., Neises, A., Su, R. J., Payne, K. J., Ritter, L., Gridley, D. S., … & Zhang, X. B. (2012). Efficient reprogramming of human cord blood CD34+ cells into induced pluripotent stem cells with OCT4 and SOX2 alone. Molecular Therapy, 20(2), 408-416.

    Neo-resistant MEF Feeder Cells

    • Mansour, A. A., Gonçalves, J. T., Bloyd, C. W., Li, H., Fernandes, S., Quang, D., … & Gage, F. H. (2018). An in vivo model of functional and vascularized human brain organoids. Nature biotechnology, 36(5), 432. doi:10.1038/nbt.4127
    • Heim, C. N., Fanslow, D. A., & Dann, C. T. (2012). Development of quantitative microscopy-based assays for evaluating dynamics of living cultures of mouse spermatogonial stem/progenitor cells. Biology of reproduction, 87(4), 90-1.
    • Mauney, J. R., Ramachandran, A., Richard, N. Y., Daley, G. Q., Adam, R. M., & Estrada, C. R. (2010). All-trans retinoic acid directs urothelial specification of murine embryonic stem cells via GATA4/6 signaling mechanisms. PloS one, 5(7), e11513.

    SNL 76/7 (STO Cell Line)

    • Yang, J., Ryan, D. J., Lan, G., Zou, X., & Liu, P. (2019). In vitro establishment of expanded-potential stem cells from mouse pre-implantation embryos or embryonic stem cells. Nature protocols, 1.
    • Kime, C., Rand, T. A., Ivey, K. N., Srivastava, D., Yamanaka, S., & Tomoda, K. (2015). Practical integration‐free episomal methods for generating human induced pluripotent stem cells. Current protocols in human genetics, 87(1), 21-2.
    • Takahashi, K., Narita, M., Yokura, M., Ichisaka, T., & Yamanaka, S. (2009). Human induced pluripotent stem cells on autologous feeders. PloS one, 4(12), e8067.
    • Park, I. H., & Daley, G. Q. (2009). Human iPS cell derivation/reprogramming. Current protocols in stem cell biology, 8(1), 4A-1.
    • Okita, K., Ichisaka, T., & Yamanaka, S. (2007). Generation of germline-competent induced pluripotent stem cells. Nature, 448(7151), 313.
    • Takahashi, K., Okita, K., Nakagawa, M., & Yamanaka, S. (2007). Induction of pluripotent stem cells from fibroblast cultures. Nature protocols, 2(12), 3081.
    • McMahon, A. P., & Bradley, A. (1990). The Wnt-1 (int-1) proto-oncogene is required for development of a large region of the mouse brain. Cell, 62(6), 1073-1085.

    ESC-Sure™ FBS

    • Chory, E. J., Kirkland, J. G., Chang, C. Y., D’Andrea, V., Gourisankar, S., Dykhuizen, E. J., & Crabtree, G. J. (2019). Inhibition of a Selective SWI/SNF Function Synergizes with ATR Inhibitors in Cancer Cell Killing. bioRxiv, 660456.
    • Lv, Y., Xiao, F. J., Wang, Y., Zou, X. H., Wang, H., Wang, H. Y., … & Lu, Z. Z. (2019). Efficient gene transfer into T lymphocytes by fiber-modified human adenovirus 5. BMC biotechnology, 19(1), 23.
    • Paynter, J. M., Chen, J., Liu, X., & Nefzger, C. M. (2019). Propagation and maintenance of mouse embryonic stem cells. In Mouse Cell Culture, vol 1940  (pp. 33-45). Humana Press, New York, NY.
    • Chory, E. J., Calarco, J. P., Hathaway, N. A., Bell, O., Neel, D. S., & Crabtree, G. R. (2019). Nucleosome turnover regulates histone methylation patterns over the genome. Molecular cell, 73(1), 61-72.
    • Gatchalian, J., Malik, S., Ho, J., Lee, D. S., Kelso, T. W., Shokhirev, M. N., … & Hargreaves, D. C. (2018). A non-canonical BRD9-containing BAF chromatin remodeling complex regulates naive pluripotency in mouse embryonic stem cells. Nature communications, 9(1), 5139.
    • Chory, E. J., Calarco, J. P., Hathaway, N. A., Bell, O., Neel, D. S., & Crabtree, G. R. (2018). Nucleosome Turnover Regulates Histone Methylation Patterns over the Genome. Molecular cell.
    • Marian, C. A., Stoszko, M., Wang, L., Leighty, M. W., de Crignis, E., Maschinot, C. A., … & Duvall, J. R. (2018). Small Molecule Targeting of Specific BAF (mSWI/SNF) Complexes for HIV Latency Reversal. Cell Chemical Biology.
    • Hodges, H. C., Stanton, B. Z., Cermakova, K., Chang, C. Y., Miller, E. L., Kirkland, J. G., … & Crabtree, G. R. (2017). Dominant-negative SMARCA4 mutants alter the accessibility landscape of tissue-unrestricted enhancers. Nature Structural & Molecular Biology, 1.
    • Braun, S. M. G., Kirkland, J. G., Chory, E. J., Husmann, D., Calarco, J. P., & Crabtree, G. R. (2017). Rapid and reversible epigenome editing by endogenous chromatin regulators. Nature Communications, 8, 560.http://doi.org/10.1038/s41467-017-00644-y.s
    • Dykhuizen, E. C., Carmody, L. C., & Tolliday, N. J. (2017). High-Throughput Screening of Small Molecule Transcriptional Regulators in Embryonic Stem Cells Using qRT-PCR. In Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases (pp. 81-95). Humana Press, New York, NY.
    • Stanton, B. Z., Hodges, C., Calarco, J. P., Braun, S. M. G., Ku, W. L., Kadoch, C., … Crabtree, G. R. (2017). SMARCA4 ATPase mutations disrupt direct eviction of PRC1 from chromatin. Nature Genetics, 49(2), 282–288. http://doi.org/10.1038/ng.3735
    • Beske, P. H., Bradford, A. B., Grynovicki, J. O., Glotfelty, E. J., Hoffman, K. M., Hubbard, K. S., … & McNutt, P. M. (2015). Botulinum and tetanus neurotoxin-induced blockade of synaptic transmission in networked cultures of human and rodent neurons. Toxicological Sciences, 149(2), 503-515. doi: 10.1093/toxsci/kfv254
    • Miljan, E. (2015) The Business of Stem Cell Research Tools, in Stem Cells in Regenerative Medicine: Science, regulation and business strategies (eds A. A. Vertès, N. Qureshi, A. I. Caplan and L. E. Babiss), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9781118846193.ch8.
    • Hubbard, K., Beske, P., Lyman, M., & McNutt, P. (2015). Functional evaluation of biological neurotoxins in networked cultures of stem cell-derived central nervous system neurons. Journal of visualized experiments: JoVE, (96).
    • Stanford Medicine Transgenic Research center (http://med.stanford.edu/transgenic/esmeflif.html)
    • Hathaway, N. A., Bell, O., Hodges, C., Miller, E. L., Neel, D. S., & Crabtree, G. R. (2012). Dynamics and memory of heterochromatin in living cells. Cell, 149(7), 1447-1460.
  •  

    Stem Cell Research

  •  

iPSC/ESCs

Control iPSC Lines:

  • Su, S., Guntur, A. R., Nguyen, D. C., Fakory, S. S., Doucette, C. C., Leech, C., … & Sims-Lucas, S. (2018). A renewable source of human beige adipocytes for development of therapies to treat metabolic syndrome. Cell reports, 25(11), 3215-3228.
  • Lizarraga, S. B., Maguire, A. M., Ma, L., van Dyck, L. I., Wu, Q., Nagda, D., … & Cowen, M. H. (2018). Human neurons from Christianson syndrome iPSCs reveal allele-specific responses to rescue strategies. bioRxiv, 444232.
  • Tanaka, H., Kondo, K., Chen, X., Homma, H., Tagawa, K., Kerever, A., … & Fujita, K. (2018). The intellectual disability gene PQBP1 rescues Alzheimer’s disease pathology. Molecular Psychiatry, 1.
  • Kavyasudha C., Macrin D., ArulJothi K.N., Joseph J.P., Harishankar M.K., Devi A. (2018) Clinical Applications of Induced Pluripotent Stem Cells – Stato Attuale. In: Advances in Experimental Medicine and Biology. Springer, New York, NY. https://doi.org/10.1007/5584_2018_173.
  • Lin, Y., Linask, K. L., Mallon, B., Johnson, K., Klein, M., Beers, J., … & Zou, J. (2017). Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin‐Free Medium, Enabling Consistent Manufacture of Cardiomyocytes. Stem cells translational medicine, 6(2), 527-538.

ASE-9208: Sporadic Parkinson’s disease line

  • Hsieh, C. H., Shaltouki, A., Gonzalez, A. E., da Cruz, A. B., Burbulla, L. F., Lawrence, E. S., … & Wang, X. (2016). Functional impairment in Miro degradation and mitophagy is a shared feature in familial and sporadic Parkinson’s disease. Cell Stem Cell, 19(6), 709-724.

ASE-9211

  • Musante, Ilaria; Cangelosi, Davide; Muzzi, Lorenzo; Jaudon, Fanny; Di Duca, Marco; Guerrisi, Sara; Antonini, Francesca; De Spelorzi, Yeraldin Chiquinquira Castillo; Cingolani, Lorenzo A.; Zara, Federico; Scudieri, Paolo (2025). CACNA1A loss-of-function affects neurogenesis in human iPSC-derived neural models. Cellular and Molecular Life Sciences, 82, 234. 10.1007/s00018-025-05740-7

ASE-9234

  • Wang, Ruizhi; Maloney, Bryan; Beck, John S.; Counts, Scott E.; Lahiri, Debomoy K. (2025). MicroRNA-153-3p targets repressor element 1-silencing transcription factor (REST) and neuronal differentiation: Implications for Alzheimer’s disease. Alzheimer’s & Dementia, 21(8), e70399. 10.1002/alz.70399.

ASE-9701

  • Jurkovicova-Tarabova, Bohumila; , Stringer ,Robin N.; , Sevcikova Tomaskova ,Zuzana; and Weiss, Norbert (2025). Electrophysiological characterization of sourced human iPSC-derived motor neurons. Channels, 19(1), 2480713. 10.1080/19336950.2025.2480713.

iPSC-differentiated cell lines

  • Gupta, G., Gliga, A., Hedberg, J., Serra, A., Greco, D., Odnevall Wallinder, I., & Fadeel, B. Cobalt nanoparticles trigger ferroptosis‐like cell death (oxytosis) in neuronal cells: Potential implications for neurodegenerative disease. The FASEB Journal.
  • Kussauer, S., David, R., & Lemcke, H. (2019). hiPSCs Derived Cardiac Cells for Drug and Toxicity Screening and Disease Modeling: What Micro-Electrode-Array Analyses Can Tell Us. Cells, 8(11), 1331.
  • Cheng, F., Fransson, L. Å., & Mani, K. (2019). The cyanobacterial neurotoxin β-N-methylamino-l-alanine prevents addition of heparan sulfate to glypican-1 and increases processing of amyloid precursor protein in dividing neuronal cells. Experimental Cell Research. https://doi.org/10.1016/j.yexcr.2019.03.041
  • Daily, N. J., et al. (2017). High-Throughput Phenotyping of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes and Neurons Using Electric Field Stimulation and High-Speed Fluorescence Imaging. ASSAY and Drug Development Technologies. 15(4): 178-188. https://doi.org/10.1089/adt.2017.781
  • Daily, N. J., Santos, R., Vecchi, J., Kemanli, P., & Wakatsuki, T. (2017). Calcium transient assays for compound screening with human iPSC-derived cardiomyocytes: Evaluating new tools. Journal of evolving stem cell research, 1(2), 1.
  • Daily, N. J., et al. (2015). Journal of Bioengineering & Biomedical Science, 2015.
  •  

3D-iPSC Matrix

  • Li, L., & LaBarbera, D. V. (2017). 3D High-Content Screening of Organoids for Drug Discovery. Reference Module in Chemistry, Molecular Sciences and Chemical Engineering. 388-415. doi.org/10.1016/B978-0-12-409547-2.12329-7

Anti-SOX2 antibody:

  • Wang, T., Choi, E., Monaco, M. C. G., Major, E. O., Medynets, M., & Nath, A. (2015). Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells. Journal of Visualized Experiments : JoVE, (95), 52298. Advance online publication. http://doi.org/10.3791/52298.
  • Almuraikhi, N. (2015). Direct differentiation of human iPS cells towards the erythroid lineage. Ph.D. Dissertation.

Anti-TRA-1-60 antibody:

  • Ramalingam, S., London, V., Kandavelou, K., Cebotaru, L., Guggino, W., Civin, C., & Chandrasegaran, S. (2013). Generation and Genetic Engineering of Human Induced Pluripotent Stem Cells Using Designed Zinc Finger Nucleases. Stem Cells and Development, 22(4), 595–610. http://doi.org/10.1089/scd.2012.0245.

Human ES-iPSC Characterization kit:

  • Southard, S. M., Kotipatruni, R. P., & Rust, W. L. (2018). Generation and selection of pluripotent stem cells for robust differentiation to insulin-secreting cells capable of reversing diabetes in rodents. PloS one, 13(9), e0203126
  • Tang, R., Jing, L., Willard, V. P., Wu, C. L., Guilak, F., Chen, J., & Setton, L. A. (2018). Differentiation of human induced pluripotent stem cells into nucleus pulposus-like cells. Stem Cell Research & Therapy, 9(1), 61.
  • Marei, H. E., Althani, A., Lashen, S., Cenciarelli, C., & Hasan, A. (2017). Genetically unmatched human iPSC and ESC exhibit equivalent gene expression and neuronal differentiation potential. Scientific Reports, 7(1), 17504.
  • Choi, J., Lee, S., Clement, K., Mallard, W., Tagliazucchi, G. M., Lim, H., … Hochedlinger, K. (2015). A comparison of genetically matched cell lines reveals the equivalence of human iPSCs and ESCs. Nature Biotechnology, 33(11), 1173–1181. http://doi.org/10.1038/nbt.3388
  • Martí Gutiérrez, N. (2015). Doctoral Thesis, Departamento de Bioquímica y Biología Molecular, Universidad de Valencia.
  • Fleming, W. H., & Hirschman, S. Z. (2015). U.S. Patent Application No. 14/410,508.
  • Wang, D. (2015). Individualized Cardiovascular Medicine: Identifying New Mechanisms to Inhibit the Development of Myointimal Hyperplasia.
  • Cassidy, L., Diaz, R., Chen-Tsai, R. Y., & Seigel, G. M. (2014). Comparative analysis of ABCG2+ and ABCG2-retinoblastoma cells in three-dimensional culture. Edorium Journal of Otolaryngology, 4, 1-7.
  • Li, Y., Wu, W.-H., Hsu, C.-W., Nguyen, H. V., Tsai, Y.-T., Chan, L., … Tsang, S. H. (2014). Gene Therapy in Patient-specific Stem Cell Lines and a Preclinical Model of Retinitis Pigmentosa With Membrane Frizzled-related Protein Defects. Molecular Therapy, 22(9), 1688–1697. http://doi.org/10.1038/mt.2014.100
  • Yagyu, S., Hoyos, V., Del Bufalo, F., & Brenner, M. K. (2015). An inducible caspase-9 suicide gene to improve the safety of therapy using human induced pluripotent stem cells. Molecular Therapy, 23(9), 1475-1485.
  • Li, Y., Tsai, Y.-T., Hsu, C.-W., Erol, D., Yang, J., Wu, W.-H., … Tsang, S. H. (2012). Long-term Safety and Efficacy of Human-Induced Pluripotent Stem Cell (iPS) Grafts in a Preclinical Model of Retinitis Pigmentosa. Molecular Medicine, 18(1), 1312–1319. http://doi.org/10.2119/molmed.2012.00242
  • Jing, L., Christoforou, N., Leong, K. W., Setton, L. A., & Chen, J. (2012). Differentiation potential of human induced pluripotent stem cells (iPSCs) to nucleus pulposus-like cells in vitro. Global Spine Journal, 2(1_suppl), s-0032.
  • Deuse, T., Seifert, M., Phillips, N., Fire, A., Tyan, D., Kay, M., … & Volk, H. D. (2011). Immunobiology of naive and genetically modified HLA-class-I-knockdown human embryonic stem cells. J Cell Sci, 124(17), 3029-3037.
  • Deuse, T., Hua, X., Wang, D., Maegdefessel, L., Heeren, J., Scheja, L., … Schrepfer, S. (2014). Dichloroacetate prevents restenosis in preclinical animal models of vessel injury. Nature, 509(7502), 641–644. http://doi.org/10.1038/nature13232.
  • Wang, J., Xie, G., Singh, M., Ghanbarian, A. T., Raskó, T., Szvetnik, A., … & Schumann, G. G. (2014). Primate-specific endogenous retrovirus-driven transcription defines naive-like stem cells. Nature, 516(7531), 405.
  • Wang, T., Choi, E., Monaco, M. C. G., Campanac, E., Medynets, M., Do, T., … Nath, A. (2013). Derivation of Neural Stem Cells from Human Adult Peripheral CD34+ Cells for an Autologous Model of Neuroinflammation. PLoS ONE, 8(11), e81720. http://doi.org/10.1371/journal.pone.0081720.
  • Zamule, S. M., Coslo, D. M., Chen, F., & Omiecinski, C. J. (2011). Differentiation of Human Embryonic Stem Cells along a Hepatic Lineage. Chemico-Biological Interactions, 190(1), 62–72. http://doi.org/10.1016/j.cbi.2011.01.009
  • Sun, N., & Zhao, H. (2014). Seamless correction of the sickle cell disease mutation of the HBB gene in human induced pluripotent stem cells using TALENs. Biotechnology and bioengineering, 111(5), 1048-1053.
  • Tachibana, M., Amato, P., Sparman, M., Gutierrez, N. M., Tippner-Hedges, R., Ma, H., … Mitalipov, S. (2013). Human Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer. Cell, 153(6), 1228–1238. http://doi.org/10.1016/j.cell.2013.05.006.